ArticleThe Journal of biological chemistry2015
Dimethylated H3K27 Is a Repressive Epigenetic Histone Mark in the Protist Entamoeba histolytica and Is Significantly Enriched in Genes Silenced via the RNAi Pathway.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.Trends in parasitology · 2025Review
- RNA-directed DNA methylation mutants reduce histone methylation at the paramutated maize booster1 enhancer.Plant physiology · 2024Article
- Advances in Protozoan Epigenetic Targets and Their Inhibitors for the Development of New Potential Drugs.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Article
- Growth and Genetic Manipulation of Entamoeba histolytica.Current protocols · 2022Article
- The state of the art of extracellular vesicle research in protozoan infection.Frontiers in genetics · 2022Review
- Are Metabolites From the Gut Microbiota Capable of Regulating Epigenetic Mechanisms in the Human ParasiteFrontiers in cell and developmental biology · 2022Review
- Establishment of quantitative RNAi-based forward genetics in Entamoeba histolytica and identification of genes required for growth.PLoS pathogens · 2021Article
- Article
- Effect of periostin silencing on Runx2, RANKL and OPG expression in osteoblasts.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2021Article
- Possible role played by the SINE2 element in gene regulation, as demonstrated by differential processing and polyadenylation in avirulent strains of E. histolytica.Antonie van Leeuwenhoek · 2021Article
- Identification of oligo-adenylated small RNAs in the parasite Entamoeba and a potential role for small RNA control.BMC genomics · 2020Article
- Article
- Telomeric Repeat-Binding Factor Homologs inFrontiers in cellular and infection microbiology · 2018Article
- Genetic, metabolomic and transcriptomic analyses of the de novo L-cysteine biosynthetic pathway in the enteric protozoan parasite Entamoeba histolytica.Scientific reports · 2017Article
- The therapeutic potential of epigenetic manipulation during infectious diseases.Pharmacology & therapeutics · 2016Review
- Development of RNA Interference Trigger-Mediated Gene Silencing in Entamoeba invadens.Infection and immunity · 2016Article
- Technical advances in trigger-induced RNA interference gene silencing in the parasite Entamoeba histolytica.International journal for parasitology · 2016Article
- Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica.Parasites & vectors · 2016Article
- Recent advances inF1000Research · 2016Review
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
RNA interference (RNAi) is a fundamental biological process that plays a crucial role in regulation of gene expression in many organisms. Transcriptional gene silencing (TGS) is one of the important nuclear roles of RNAi. Our previous data show that Entamoeba histolytica has a robust RNAi pathway that links to TGS via Argonaute 2-2 (Ago2-2) associated 27-nucleotide small RNAs with 5'-polyphosphate termini. Here, we report the first repressive histone mark to be identified in E. histolytica, dimethylation of H3K27 (H3K27Me2), and demonstrate that it is enriched at genes that are silenced by RNAi-mediated TGS. An RNAi-silencing trigger can induce H3K27Me2 deposits at both episomal and chromosomal loci, mediating gene silencing. Our data support two phases of RNAi-mediated TGS: an active silencing phase where the RNAi trigger is present and both H3K27Me2 and Ago2-2 concurrently enrich at chromosomal loci; and an established silencing phase in which the RNAi trigger is removed, but gene silencing with H3K27Me2 enrichment persist independently of Ago2-2 deposition. Importantly, some genes display resistance to chromosomal silencing despite induction of functional small RNAs. In those situations, the RNAi-triggering plasmid that is maintained episomally gets partially silenced and has H3K27Me2 enrichment, but the chromosomal copy displays no repressive histone enrichment. Our data are consistent with a model in which H3K27Me2 is a repressive histone modification, which is strongly associated with transcriptional repression. This is the first example of an epigenetic histone modification that functions to mediate RNAi-mediated TGS in the deep-branching eukaryote E. histolytica.
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