Evidence map›Paper›PMID 26149575›Full record

ArticlePlant physiology2015

Rate Motifs Tune Auxin/Indole-3-Acetic Acid Degradation Dynamics.

Britney L Moss, Haibin Mao, Jessica M Guseman, Thomas R Hinds, Antje Hellmuth, Marlies Kovenock, Anisa Noorassa, Amy Lanctot, Luz Irina A Calderón Villalobos, Ning Zheng and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 78 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Genome-Wide Identification and Analysis of the Aux/IAA Gene Family inInternational journal of molecular sciences · 2024
    Article
  7. Article
  8. Intrinsic disorder and conformational coexistence in auxin coreceptors.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  9. Article
  10. CPR5-mediated nucleo-cytoplasmic localization of IAA12 and IAA19 controls lateral root development during abiotic stress.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  11. Review
  12. Article
  13. Review
  14. The Systems and Synthetic Biology of Auxin.Cold Spring Harbor perspectives in biology · 2022
    Review
  15. Structural Aspects of Auxin Signaling.Cold Spring Harbor perspectives in biology · 2022
    Review
  16. Article
  17. Article
  18. No Time for Transcription-Rapid Auxin Responses in Plants.Cold Spring Harbor perspectives in biology · 2021
    Review
  19. Review
  20. Improving architectural traits of maize inflorescences.Molecular breeding : new strategies in plant improvement · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Britney L MossDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Haibin MaoDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Jessica M GusemanDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Thomas R HindsDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Antje HellmuthDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Marlies KovenockDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Anisa NoorassaDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Amy LanctotDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Luz Irina A Calderón VillalobosDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Ning ZhengDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.).
Jennifer L NemhauserDepartments of Biology (B.L.M., J.M.G., M.K., A.N., A.L., J.L.N.) and Pharmacology (H.M., T.R.H., N.Z.), University of Washington, Seattle, Washington 98195;Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (A.H., L.I.A.C.V.); andHoward Hughes Medical Institute, Chevy Chase, Maryland 20815 (N.Z.) jn7@uw.edu.
University of Washington · USLeibniz Institute of Plant Biochemistry · DE

Funding

F-box proteins: deregulated cell cycle control and proteolysis in cancerR37CA076584 · NCI · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI PAGANO, MICHELE · 2006 to 2016
$4.7M
F-Box Proteins: Deregulated Cell Cycle Control and Proteolysis in CancerR01CA076584 · NCI · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI PAGANO, MICHELE · 1998 to 2021
$4.5M
Control of protein degradation dynamics in the auxin responseR01GM107084 · NIGMS · UNIVERSITY OF WASHINGTON · PI NEMHAUSER, JENNIFER L · 2014 to 2022
$2.4M
Determinants of Protein Degradation Dynamics in Cell SignalingF32CA180514 · NCI · UNIVERSITY OF WASHINGTON · PI MOSS, BRITNEY LANE · 2013 to 2015
$111k
Howard Hughes Medical InstituteNCI NIH HHS F32 CA180514NCI NIH HHS F32CA180514NCI NIH HHS R01 CA076584NCI NIH HHS R01-CA076584NCI NIH HHS R37 CA076584NIGMS NIH HHS R01 GM107084NIGMS NIH HHS R01-GM107084
6 · The paper itself

Abstract

Ubiquitin-mediated protein degradation is a common feature in diverse plant cell signaling pathways; however, the factors that control the dynamics of regulated protein turnover are largely unknown. One of the best-characterized families of E3 ubiquitin ligases facilitates ubiquitination of auxin (aux)/indole-3-acetic acid (IAA) repressor proteins in the presence of auxin. Rates of auxin-induced degradation vary widely within the Aux/IAA family, and sequences outside of the characterized degron (the minimum region required for auxin-induced degradation) can accelerate or decelerate degradation. We have used synthetic auxin degradation assays in yeast (Saccharomyces cerevisiae) and in plants to characterize motifs flanking the degron that contribute to tuning the dynamics of Aux/IAA degradation. The presence of these rate motifs is conserved in phylogenetically distant members of the Arabidopsis (Arabidopsis thaliana) Aux/IAA family, as well as in their putative Brassica rapa orthologs. We found that rate motifs can act by enhancing interaction between repressors and the E3, but that this is not the only mechanism of action. Phenotypes of transgenic plants expressing a deletion in a rate motif in IAA28 resembled plants expressing degron mutations, underscoring the functional relevance of Aux/IAA degradation dynamics in regulating auxin responses.

Indexed as

ProteolysisAmino Acid MotifsIndoleacetic AcidsPlant ProteinsPlant RootsProtein BindingProtein Structure, Tertiaryindoleacetic acidIndoleacetic AcidsPlant Proteins

Identifiers

PMID26149575
PMCPMC4577399
OpenAlexW2108272661

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.