ArticleActa tropica2015
Correlation of serum and dried blood spot results for quantitation of Schistosoma circulating anodic antigen: a proof of principle.
Article in Acta tropica, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Refining Diagnosis ofFrontiers in immunology · 2018Trial
- Community-Based Screening for HIV and Viral Hepatitis Using Dried Blood Spots in a Migrant Population in Spain: The HEPINMIGRA Study.AIDS and behavior · 2026Article
- Schistosomiasis in Western Lake Turkana, Kenya: An Exploratory Serosurvey and Validation of Dried Blood Spots for Field Studies.Tropical medicine and infectious disease · 2026Article
- Impact of -20 C° cryopreservation on serum factors from schistosomiasis patients at different storage durations: insights into serum bio-banking.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Article
- The Use of Dried Matrix Spots as an Alternative Sampling Technique for Monitoring Neglected Tropical Diseases.Pathogens (Basel, Switzerland) · 2024Review
- Detecting twoFrontiers in parasitology · 2024Article
- UCP-LF and other assay methods for schistosome circulating anodic antigen between 1978 and 2022.Biology methods & protocols · 2023Review
- Dried blood sample analysis by antibody array across the total testing process.Scientific reports · 2021Article
- Detection of Schistosoma mansoni DNA using polymerase chain reaction from serum and dried blood spot card samples of an adult population in North-western Tanzania.Infectious diseases of poverty · 2021Article
- Circulating Anodic Antigen (CAA): A Highly Sensitive Diagnostic Biomarker to Detect ActiveThe American journal of tropical medicine and hygiene · 2020Review
- HIV-seroconversion among HIV-1 serodiscordant married couples in Tanzania: a cohort study.BMC infectious diseases · 2019Article
- Impact of schistosome infection on long-term HIV/AIDS outcomes.PLoS neglected tropical diseases · 2018Article
- Utilizing the ultrasensitive Schistosoma up-converting phosphor lateral flow circulating anodic antigen (UCP-LF CAA) assay for sample pooling-strategies.Infectious diseases of poverty · 2017Article
- Effects of schistosomiasis on susceptibility to HIV-1 infection and HIV-1 viral load at HIV-1 seroconversion: A nested case-control study.PLoS neglected tropical diseases · 2017Article
- Comparison ofFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Circulating anodic antigen (CAA) testing is a powerful, increasingly-used tool for diagnosis of active schistosome infection. We sought to determine the feasibility and reliability of measuring CAA in blood spots collected on Whatman 903 protein saver cards, which are the predominant filter papers used worldwide for dried blood spot (DBS) research and clinical care. CAA was eluted from blood spots collected from 19 individuals onto Whatman 903 cards in Mwanza, Tanzania, and the assay was optimized to achieve CAA ratios comparable to those obtained from the spots' corresponding serum samples. The optimized assay was then used to determine the correlation of serum samples (n=16) with DBS from cards that had been stored for 8 years at ambient temperature. Using a DBS volume equivalent to approximately four times the quantity of serum, CAA testing in DBS had a sensitivity of 76% and a specificity of 79% compared to CAA testing in serum. CAA testing was reliable in samples eluted from Whatman 903 cards that had been stored for 8 years at ambient temperature. The overall kappa coefficient was 0.53 (standard error 0.17, p<0.001). We conclude that CAA can be reliably and accurately measured in DBS collected onto the filter paper that is most commonly used for clinical care and research, and that can be stored from prolonged periods of time. This finding opens new avenues for future work among more than 700million individuals living in areas worldwide in which schistosomes are endemic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.