ArticleScientific reports2015
Scaffolding of a bacterial genome using MinION nanopore sequencing.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Draft genome sequence ofMicrobiology resource announcements · 2026Article
- MitoCOMON: whole mitochondrial DNA sequencing by primer design and long overlapping amplicon assembly.BMC genomics · 2025Article
- The quality and detection limits of mitochondrial heteroplasmy by long read nanopore sequencing.Scientific reports · 2024Article
- Article
- The Applications of Nanopore Sequencing Technology in Animal and Human Virus Research.Viruses · 2024Review
- Proof of concept for multiplex amplicon sequencing for mutation identification using the MinION nanopore sequencer.Scientific reports · 2022Article
- Application and Challenge of 3rd Generation Sequencing for Clinical Bacterial Studies.International journal of molecular sciences · 2022Review
- Functional characterization of prokaryotic dark matter: the road so far and what lies ahead.Current research in microbial sciences · 2022Review
- Invasive atypical non-typhoidalMicrobial genomics · 2021Article
- Is Oxford Nanopore sequencing ready for analyzing complex microbiomes?FEMS microbiology ecology · 2021Article
- Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination.Antibiotics (Basel, Switzerland) · 2020Article
- Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus.Scientific reports · 2020Article
- Longshot enables accurate variant calling in diploid genomes from single-molecule long read sequencing.Nature communications · 2019Article
- Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing.BMC genomics · 2019Article
- High-throughput long paired-end sequencing of a Fosmid library by PacBio.Plant methods · 2019Article
- Rapid metagenomics analysis of EMS vehicles for monitoring pathogen load using nanopore DNA sequencing.PloS one · 2019Article
- Nanopore sequencing of drug-resistance-associated genes in malaria parasites, Plasmodium falciparum.Scientific reports · 2018Article
- Systematic and stochastic influences on the performance of the MinION nanopore sequencer across a range of nucleotide bias.Scientific reports · 2018Article
- Comprehensive benchmarking and ensemble approaches for metagenomic classifiers.Genome biology · 2017Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Second generation sequencing has revolutionized genomic studies. However, most genomes contain repeated DNA elements that are longer than the read lengths achievable with typical sequencers, so the genomic order of several generated contigs cannot be easily resolved. A new generation of sequencers offering substantially longer reads is emerging, notably the Pacific Biosciences (PacBio) RS II system and the MinION system, released in early 2014 by Oxford Nanopore Technologies through an early access program. The latter has highly advantageous portability and sequences samples by measuring changes in ionic current when single-stranded DNA molecules are translocated through nanopores. We show that the MinION system produces long reads with high mapability that can be used for scaffolding bacterial genomes, despite currently producing substantially higher error rates than PacBio reads. With further development we anticipate that MinION will be useful not only for assembling genomes, but also for rapid detection of organisms, potentially in the field.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.