ArticleScientific reports2015
Primary microRNA processing is functionally coupled to RNAP II transcription in vitro.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Bioinformatics analysis to explore the potential prognostic utility of hsa-miR-103a-3p in head and neck squamous cell carcinoma.Scientific reports · 2025Article
- Gamma-retrovirus design for efficacious microRNA-mediated gene knock-down and protein co-expression without compromising titers.NAR molecular medicine · 2025Article
- Regulation of the microprocessor by post-translational modifications.Frontiers in cell and developmental biology · 2025Review
- A transcription-independent role for HIF-1α in modulating microprocessor assembly.Nucleic acids research · 2024Article
- Role of the proline-rich disordered domain of DROSHA in intronic microRNA processing.Genes & development · 2023Article
- Chromatin-associated microprocessor assembly is regulated by the U1 snRNP auxiliary protein PRP40.The Plant cell · 2022Article
- The Implications of Noncoding RNAs in the Evolution and Progression of Nonalcoholic Fatty Liver Disease (NAFLD)-Related HCC.International journal of molecular sciences · 2022Review
- The Potential Role of miRNAs as Predictive Biomarkers in Neurodevelopmental Disorders.Journal of molecular neuroscience : MN · 2021Review
- Multifaceted Regulation of MicroRNA Biogenesis: Essential Roles and Functional Integration in Neuronal and Glial Development.International journal of molecular sciences · 2021Review
- Emerging Roles and Potential Applications of Non-Coding RNAs in Glioblastoma.International journal of molecular sciences · 2020Review
- Novel Nuclear Functions of Arabidopsis ARGONAUTE1: Beyond RNA Interference.Plant physiology · 2019Review
- Regulation of microRNA biogenesis and its crosstalk with other cellular pathways.Nature reviews. Molecular cell biology · 2019Review
- The Emerging Role of microRNAs in Polyglutamine Diseases.Frontiers in molecular neuroscience · 2019Article
- In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-Way System for RNAP II Transcription/Splicing/microRNA Processing.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Analysis of primary microRNA loci from nascent transcriptomes reveals regulatory domains governed by chromatin architecture.Nucleic acids research · 2017Article
- Microprocessor Recruitment to Elongating RNA Polymerase II Is Required for Differential Expression of MicroRNAs.Cell reports · 2017Article
- Evidence that C9ORF72 Dipeptide Repeat Proteins Associate with U2 snRNP to Cause Mis-splicing in ALS/FTD Patients.Cell reports · 2017Article
- HP1BP3, a Chromatin Retention Factor for Co-transcriptional MicroRNA Processing.Molecular cell · 2016Article
- Splicing noncoding RNAs from the inside out.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Previous studies in vivo reported that processing of primary microRNA (pri-miRNA) is coupled to transcription by RNA polymerase II (RNAP II) and can occur co-transcriptionally. Here we have established a robust in vivo system in which pri-miRNA is transcribed by RNAP II and processed to pre-miRNA in HeLa cell nuclear extracts. We show that both the kinetics and efficiency of pri-miRNA processing are dramatically enhanced in this system compared to that of the corresponding naked pri-miRNA. Moreover, this enhancement is general as it occurs with multiple pri-miRNAs. We also show that nascent pri-miRNA is efficiently processed before it is released from the DNA template. Together, our work directly demonstrates that transcription and pri-miRNA processing are functionally coupled and establishes the first in vivo model systems for this functional coupling and for co-transcriptional processing.
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