ArticleClinical and experimental immunology2015
Similar activation state of neutrophils in sputum of asthma patients irrespective of sputum eosinophilia.
Article in Clinical and experimental immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Surface expression of CD63 and HLA-DR in circulating eosinophils correlates with improved clinical control after treatment optimization in asthma.Scientific reports · 2026Trial
- Asthma endotypes and theratypes.Chinese medical journal pulmonary and critical care medicine · 2026Review
- Severe Dry Cough Followed by Eosinophilia Without Pulmonary Infiltrates After Dupilumab Treatment in Patients with Severe Asthma: A Series of Five Cases.Journal of inflammation research · 2025Article
- Article
- Improved cytometric analysis of untouched lung leukocytes by enzymatic liquefaction of sputum samples.Biological procedures online · 2022Article
- Article
- Elevation of activated neutrophils in chronic rhinosinusitis with nasal polyps.The Journal of allergy and clinical immunology · 2022Article
- Enhanced Glycolysis Is Required for Antileishmanial Functions of Neutrophils Upon Infection WithFrontiers in immunology · 2021Article
- The Effects of Indoor Pollutants Exposure on Allergy and Lung Inflammation: An Activation State of Neutrophils and Eosinophils in Sputum.International journal of environmental research and public health · 2020Article
- Safeguard function of PU.1 shapes the inflammatory epigenome of neutrophils.Nature immunology · 2019Article
- Children with Neutrophil-Predominant Severe Asthma Have Proinflammatory Neutrophils With Enhanced Survival and Impaired Clearance.The journal of allergy and clinical immunology. In practice · 2019Article
- Article
- Severe bronchial asthma in children: a review of novel biomarkers used as predictors of the disease.Journal of asthma and allergy · 2018Review
- Effects of treatment changes on asthma phenotype prevalence and airway neutrophil function.BMC pulmonary medicine · 2017Article
- Eosinophil Activation Status in Separate Compartments and Association with Asthma.Frontiers in medicine · 2017Review
- Leukotriene B4 levels in sputum from asthma patients.ERJ open research · 2016Article
- How Neutrophils Shape Adaptive Immune Responses.Frontiers in immunology · 2015Review
- Possible Noninvasive Biomarker of Eosinophilic Esophagitis: Clinical and Experimental Evidence.Case reports in gastroenterologyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inflammatory phenotypes of asthma are associated with differences in disease characteristics. It is unknown whether these inflammatory phenotypes are reflected by the activation status of neutrophils in blood and sputum. We obtained peripheral blood and induced sputum from 21 asthma patients and stratified our samples based on sputum eosinophilia resulting in two groups (>3% eosinophils: n = 13, <3%: n = 8). Eosinophils and neutrophils from blood and sputum were analysed for expression of activation and degranulation markers by flow cytometry. Data were analysed by both classical, non-parametric statistics and a multi-dimensional approach, using principal component analysis (PCA). Patients with sputum eosinophilia were characterized by increased asthma control questionnaire (ACQ) scores and blood eosinophil counts. Both sputum neutrophils and eosinophils displayed an activated and degranulated phenotype compared to cells obtained from blood. Specifically, degranulation of all granule types was detected in sputum cells, combined with an increased expression of the activation markers (activated) Mac-1 (CD11b), programmed death ligand 1 (PD-L1) (CD274) and a decreased expression of CD62L. CD69 expression was only increased on sputum eosinophils. Surface marker expression of neutrophils was similar in the presence or absence of eosinophilia, either by single or multi-dimensional analysis. Sputum neutrophils were highly activated and degranulated irrespective of sputum eosinophilia. Therefore, we conclude that differences in granulocyte activation in sputum and/or blood are not associated with clinical differences in the two groups of asthma patients. The finding of PD-L1 expression on sputum granulocytes suggests an immunomodulatory role of these cells in the tissue.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.