ArticleScientific reports2015
Green to red photoconversion of GFP for protein tracking in vivo.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Signal control during tissue regeneration in adult animals.Nature reviews. Molecular cell biology · 2026Review
- Advantages and Limitations of Photoconvertible Probes to Study Subcellular Dynamics in Epithelial Cells.Biology of the cell · 2025Article
- Structural origin and rational development of bright red noncanonical variants of green fluorescent protein.Physical chemistry chemical physics : PCCP · 2023Article
- Correlation of vacuole morphology with stomatal lineage development by whole-cell electron tomography.Plant physiology · 2022Article
- Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A general strategy to red-shift green fluorescent protein-based biosensors.Nature chemical biology · 2020Article
- Spontaneous and Induced Animal Models for Cancer Research.Diagnostics (Basel, Switzerland) · 2020Review
- A General Mechanism of Green-to-Red Photoconversions of GFP.Frontiers in molecular biosciences · 2020Article
- Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission.Scientific reports · 2019Article
- A novel myelin protein zero transgenic zebrafish designed for rapid readout of in vivo myelination.Glia · 2019Article
- Repeat-associated non-AUG translation in C9orf72-ALS/FTD is driven by neuronal excitation and stress.EMBO molecular medicine · 2019Article
- Role of green fluorescent proteins and their variants in development of FRET-based sensors.Journal of biosciences · 2018Review
- Transgenic Mouse Models in Cancer Research.Frontiers in oncology · 2018Review
- Protein bodies: how the ER deals with high accumulation of recombinant proteins.Plant biotechnology journal · 2017Article
- The rise of photoresponsive protein technologies applicationsF1000Research · 2017Review
- Cellular GFP Toxicity and Immunogenicity: Potential Confounders in in Vivo Cell Tracking Experiments.Stem cell reviews and reports · 2016Review
- Genetically encoded indicators of neuronal activity.Nature neuroscience · 2016Review
- Protein Bodies in Leaves Exchange Contents through the Endoplasmic Reticulum.Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A variety of fluorescent proteins have been identified that undergo shifts in spectral emission properties over time or once they are irradiated by ultraviolet or blue light. Such proteins are finding application in following the dynamics of particular proteins or labelled organelles within the cell. However, before genes encoding these fluorescent proteins were available, many proteins have already been labelled with GFP in transgenic cells; a number of model organisms feature collections of GFP-tagged lines and organisms. Here we describe a fast, localized and non-invasive method for GFP photoconversion from green to red. We demonstrate its use in transgenic plant, Drosophila and mammalian cells in vivo. While genes encoding fluorescent proteins specifically designed for photoconversion will usually be advantageous when creating new transgenic lines, our method for photoconversion of GFP allows the use of existing GFP-tagged transgenic lines for studies of dynamic processes in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.