ArticleJournal of cell science2015
Modulation of flagellum attachment zone protein FLAM3 and regulation of the cell shape in Trypanosoma brucei life cycle transitions.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Why Euglenozoans.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Disruption of Leishmania flagellum attachment zone architecture causes flagellum loss.Molecular microbiology · 2024Article
- Characterisation of TbSmee1 suggests endocytosis allows surface-bound cargo to enter the trypanosome flagellar pocket.Journal of cell science · 2023Article
- FAZ assembly in bloodstream formMolecular biology of the cell · 2023Article
- Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei.Nature microbiology · 2023Article
- Diversity in new flagellum tip attachment in bloodstream form African trypanosomes.Molecular microbiology · 2022Article
- Light chain 2 is a Tctex-type related axonemal dynein light chain that regulates directional ciliary motility in Trypanosoma brucei.PLoS pathogens · 2022Article
- Structure, function and druggability of the African trypanosome flagellum.Journal of cellular physiology · 2022Review
- Candidates for Balancing Selection in Leishmania donovani Complex Parasites.Genome biology and evolution · 2021Article
- Redistribution of FLAgellar Member 8 during the trypanosome life cycle: Consequences for cell fate prediction.Cellular microbiology · 2021Article
- APEX2 Proximity Proteomics Resolves Flagellum Subdomains and Identifies Flagellum Tip-Specific Proteins in Trypanosoma brucei.mSphere · 2021Article
- Role for the flagellum attachment zone in Leishmania anterior cell tip morphogenesis.PLoS pathogens · 2020Article
- FAZ27 cooperates with FLAM3 and ClpGM6 to maintain cell morphology inJournal of cell science · 2020Article
- Basal Body Protein TbSAF1 Is Required for Microtubule Quartet Anchorage to the Basal Bodies inmBio · 2020Article
- Touching the Surface: Diverse Roles for the Flagellar Membrane in Kinetoplastid Parasites.Microbiology and molecular biology reviews : MMBR · 2020Review
- More than Microtubules: The Structure and Function of the Subpellicular Array in Trypanosomatids.Trends in parasitology · 2019Review
- Artemisinin-resistant Leishmania parasite modulates host cell defense mechanism and exhibits altered expression of unfolded protein response genes.Parasitology research · 2019Article
- Article
- Dependency relationships between IFT-dependent flagellum elongation and cell morphogenesis inOpen biology · 2018Article
- A centriolar FGR1 oncogene partner-like protein required for paraflagellar rod assembly, but not axoneme assembly in African trypanosomes.Open biology · 2018Article
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8 authors.
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Abstract
The cell shape of Trypanosoma brucei is influenced by flagellum-to-cell-body attachment through a specialised structure - the flagellum attachment zone (FAZ). T. brucei exhibits numerous morphological forms during its life cycle and, at each stage, the FAZ length varies. We have analysed FLAM3, a large protein that localises to the FAZ region within the old and new flagellum. Ablation of FLAM3 expression causes a reduction in FAZ length; however, this has remarkably different consequences in the tsetse procyclic form versus the mammalian bloodstream form. In procyclic form cells FLAM3 RNAi results in the transition to an epimastigote-like shape, whereas in bloodstream form cells a severe cytokinesis defect associated with flagellum detachment is observed. Moreover, we demonstrate that the amount of FLAM3 and its localisation is dependent on ClpGM6 expression and vice versa. This evidence demonstrates that FAZ is a key regulator of trypanosome shape, with experimental perturbations being life cycle form dependent. An evolutionary cell biology explanation suggests that these differences are a reflection of the division process, the cytoskeleton and intrinsic structural plasticity of particular life cycle forms.
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