ArticlePloS one2015
In Vitro Evidence for Immune-Modulatory Properties of Non-Digestible Oligosaccharides: Direct Effect on Human Monocyte Derived Dendritic Cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Prebiotics and Synbiotics in Asthma: An Integrative Review of Human Trials and Murine Meta-Analysis.Nutrients · 2026Pooled it
- Fructo-Oligosaccharides Modify Human DC Maturation and Peanut-Induced Autologous T-Cell Response of Allergic PatientsFrontiers in immunology · 2020Trial
- Exploring Immune Development in Infants With Moderate to Severe Atopic Dermatitis.Frontiers in immunology · 2018Trial
- The Relationship Between Gut Microbiota and Recurrent Spontaneous Abortion.Microorganisms · 2025Review
- Targeted nutritional intervention attenuates experimental lung cancer cachexia.Journal of cachexia, sarcopenia and muscle · 2024Article
- Prebiotic diet normalizes aberrant immune and behavioral phenotypes in a mouse model of autism spectrum disorder.Acta pharmacologica Sinica · 2024Article
- Ex Vivo Immunomodulatory Effects of Lactobacillus-, Lacticaseibacillus-, and Bifidobacterium-Containing Synbiotics on Human Peripheral Blood Mononuclear Cells and Monocyte-Derived Dendritic Cells in the Context of Grass Pollen Allergy.Probiotics and antimicrobial proteins · 2023Article
- Review
- Recent Research and Application Prospect of Functional Oligosaccharides on Intestinal Disease Treatment.Molecules (Basel, Switzerland) · 2022Review
- Modulation of the Epithelial-Immune Cell Crosstalk and Related Galectin Secretion by DP3-5 Galacto-Oligosaccharides and β-3'Galactosyllactose.Biomolecules · 2022Article
- Nutritional Interventions to Prevent the Development of Atopic Diseases: A Focus on Cow's Milk Allergy.Handbook of experimental pharmacology · 2022Article
- Role of gut microbiota in the pathogenesis and treatment of diabetes mullites: Advanced research-based review.Frontiers in microbiology · 2022Review
- Galactooligosaccharide Treatment Alleviates DSS-Induced Colonic Inflammation in Caco-2 Cell Model.Frontiers in nutrition · 2022Article
- Article
- The crucial role of early-life gut microbiota in the development of type 1 diabetes.Acta diabetologica · 2021Review
- Effects of gut microbiome and environment on the development of eczema in Chinese infants.Medicine · 2020Article
- Microbiota-dependent and -independent effects of dietary fibre on human health.British journal of pharmacology · 2020Review
- Article
- Effects of inulin supplementation on intestinal barrier function and immunity in specific pathogen-free chickens with Salmonella infection.Journal of animal science · 2020Article
- Turmeric Extract: Potential Use as a Prebiotic and Anti-Inflammatory Compound?Plant foods for human nutrition (Dordrecht, Netherlands) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infant formulas containing non-digestible oligosaccharides (NDO) similar to the composition in breast milk or a combination of lactic acid bacteria (LAB) and NDO have been shown to harbor preventive effects towards immune-regulatory disorders. The aim of this study was to investigate the immune-modulatory potential of non-digestible short chain galacto- and long chain fructo-oligosaccharides (scGOS/lcFOS) mimicking the natural distribution of oligosaccharides in human breast milk in presence or absence of certain LAB strains in human monocyte derived dendritic cells (MoDC). Immature human MoDC prepared from peripheral blood of healthy non-atopic volunteers were screened in vitro after stimulation with specific scGOS/lcFOS in presence or absence of LAB. IL-10 and IL-12p70 release was analyzed after 24 hours in cell-free supernatants by enzyme-linked immunosorbent assay (ELISA). A luminex-based assay was conducted to assess further cytokine and chemokine release by MoDC. To investigate the resulting T cell response, stimulated MoDC were co-incubated with naïve T cells in allogeneic stimulation assays and intracellular Foxp3 expression, as well as immune-suppressive capacity was determined. Oligosaccharides did not induce relevant amounts of IL-12p70 production, but did promote IL-10 release by MoDC. Furthermore, scGOS/lcFOS mixtures exerted a significant enhancing effect on LAB induced IL-10 secretion by MoDC while no increase in IL-12p70 production was observed. Blocking toll like receptor (TLR)4 abrogated the increase in IL-10 in both the direct stimulation and the LAB stimulation of MoDC, suggesting that scGOS/lcFOS act via TLR4. Finally, scGOS/lcFOS-treated MoDC were shown to upregulate the number of functional suppressive Foxp3 positive T cells following allogeneic stimulation. Our results indicate anti-inflammatory and direct, microbiota independent, immune-modulatory properties of scGOS/lcFOS mixtures on human MoDC suggesting a possible induction of regulatory T cells (Tregs). The tested combinations of LAB and scGOS/lcFOS might represent a useful dietary ingredient for the maintenance of intestinal homeostasis via the induction of Tregs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.