Evidence map›Paper›PMID 26147927›Full record

ArticleChemical biology & drug design2015

Microscale Adaptation of In Vitro Transcription/Translation for High-Throughput Screening of Natural Product Extract Libraries.

Andrew N Lowell, Nicholas Santoro, Steven M Swaney, Thomas J McQuade, Pamela J Schultz, Martha J Larsen, David H Sherman

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical biology & drug design, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Overview on Strategies and Assays for Antibiotic Discovery.Pharmaceuticals (Basel, Switzerland) · 2022
    Review
  2. Activity-Based DNA-Encoded Library Screening.ACS combinatorial science · 2019
    Article
  3. Article
  4. Article
  5. Techniques for Screening Translation Inhibitors.Antibiotics (Basel, Switzerland) · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Andrew N LowellLife Sciences Institute, University of Michigan, 210 Washtenaw Ave., Ann Arbor, MI, 48109-2216, USA.
Nicholas SantoroLife Sciences Institute, University of Michigan, 210 Washtenaw Ave., Ann Arbor, MI, 48109-2216, USA.
Steven M SwaneyLife Sciences Institute, University of Michigan, 210 Washtenaw Ave., Ann Arbor, MI, 48109-2216, USA.
Thomas J McQuadeLife Sciences Institute, University of Michigan, 210 Washtenaw Ave., Ann Arbor, MI, 48109-2216, USA.
Pamela J SchultzLife Sciences Institute, University of Michigan, 210 Washtenaw Ave., Ann Arbor, MI, 48109-2216, USA.
Martha J LarsenLife Sciences Institute, University of Michigan, 210 Washtenaw Ave., Ann Arbor, MI, 48109-2216, USA.
David H ShermanLife Sciences Institute, University of Michigan, 210 Washtenaw Ave., Ann Arbor, MI, 48109-2216, USA.

Funding

of Natural Product based Drugs and Bioenergetic Materials from Costa Rican BiotaU01TW007404 · FIC · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SHERMAN, DAVID H · 2005 to 2013
$7.6M
TOTAL SYNTHESIS AND BIOSYNTHESIS OF BIOACTIVE SUBSTANCESR01CA070375 · NCI · COLORADO STATE UNIVERSITY-FORT COLLINS · PI SHERMAN, DAVID H, WILLIAMS, ROBERT MICHAEL · 1997 to 2018
$4.4M
Biosynthetic Analysis of Marine Cyanobacterial PathwaysR01CA108874 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI GERWICK, WILLIAM HENRY, SHERMAN, DAVID H · 2006 to 2015
$3.4M
FIC NIH HHS U01 TW007404NCI NIH HHS R01 CA070375NCI NIH HHS R01 CA108874
6 · The paper itself

Abstract

Novel antimicrobials that effectively inhibit bacterial growth are essential to fight the growing threat of antibiotic resistance. A promising target is the bacterial ribosome, a 2.5 MDa organelle susceptible to several biorthogonal modes of action used by different classes of antibiotics. To promote the discovery of unique inhibitors, we have miniaturized a coupled transcription/translation assay using E. coli and applied it to screen a natural product library of ~30 000 extracts. We significantly reduced the scale of the assay to 2 μL in a 1536-well plate format and decreased the effective concentration of costly reagents. The improved assay returned 1327 hits (4.6% hit rate) with %CV and Z' values of 8.5% and 0.74, respectively. This assay represents a significant advance in molecular screening, both in miniaturization and its application to a natural product extract library, and we intend to apply it to a broad array of pathogenic microbes in the search for novel anti-infective agents.

Indexed as

Anti-Infective AgentsBiological ProductsDrug Evaluation, PreclinicalEscherichia coliHigh-Throughput Screening AssaysLuciferasesMiniaturizationProtein BiosynthesisRibosomesSmall Molecule LibrariesTranscription, GeneticAnti-Infective AgentsBiological ProductsLuciferasesSmall Molecule Librariesantimicrobial screeningmicroscale high-throughput screeningnatural products extract librarytranscription/translation

Identifiers

PMID26147927
PMCPMC4888872

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.