Evidence map›Paper›PMID 26147917›Full record

ArticleNature methods2015

Multitarget super-resolution microscopy with high-density labeling by exchangeable probes.

Tai Kiuchi, Makio Higuchi, Akihiro Takamura, Masahiro Maruoka, Naoki Watanabe

Abstract read
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In one paragraph

Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.

0numbers the graph read from it
0cells of the map it votes in
78citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

78 citing papers in PubMed.

  1. Article
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  6. Color mapping of multitarget protein clusters.Structure (London, England : 1993) · 2025
    Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Article
  12. Cell-Material Interplay in Focal Adhesion Points.ACS applied materials & interfaces · 2024
    Article
  13. Article
  14. Beyond DNA: new probes for PAINT super-resolution microscopy.Chemical communications (Cambridge, England) · 2023
    Review
  15. Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach.Protein science : a publication of the Protein Society · 2023
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

18 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tai KiuchiDepartment of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan.
Makio HiguchiDepartment of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan.
Akihiro TakamuraLaboratory of Single-Molecule Cell Biology, Tohoku University Graduate School of Life Sciences, Sendai, Japan.
Masahiro MaruokaLaboratory of Single-Molecule Cell Biology, Tohoku University Graduate School of Life Sciences, Sendai, Japan.
Naoki Watanabe1] Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan. [2] Laboratory of Single-Molecule Cell Biology, Kyoto University Graduate School of Biostudies, Kyoto, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell.

Indexed as

ActinsAnimalsCytoskeletonExpressed Sequence TagsFluorescent DyesFocal AdhesionsGreen Fluorescent ProteinsHumansImage Processing, Computer-AssistedMiceMicroscopy, FluorescenceMicrotubulesOxygenPeptidesPlasmidsRatsActinsFluorescent DyesGreen Fluorescent ProteinsOxygenPeptides

Identifiers

PMID26147917

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.