ArticlePloS one2015
Structural Rigidity and Protein Thermostability in Variants of Lipase A from Bacillus subtilis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Computational Prediction of Deleterious SNPs in the GALNS Gene Implicated in Morquio A Syndrome (MPS IVA).ACS omega · 2026Article
- Exploring Bacillus species xylanases for industrial applications: screening via thermostability and reaction modelling.Journal of molecular modeling · 2024Article
- Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization.PloS one · 2024Article
- Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes.Nature communications · 2023Article
- A thermostable organic solvent-tolerant lipase fromFrontiers in microbiology · 2023Article
- Structure and Function of Redox-Sensitive Superfolder Green Fluorescent Protein Variant.Antioxidants & redox signaling · 2022Article
- Viral protein instability enhances host-range evolvability.PLoS genetics · 2022Article
- Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cell mitochondria and evolved via association of differentially adapted subunits.The Plant cell · 2022Article
- Critical assessment of structure-based approaches to improve protein resistance in aqueous ionic liquids by enzyme-wide saturation mutagenesis.Computational and structural biotechnology journal · 2022Article
- Uncoupling of Voltage- and Ligand-Induced Activation in HCN2 Channels by Glycine Inserts.Frontiers in physiology · 2022Article
- Less Unfavorable Salt Bridges on the Enzyme Surface Result in More Organic Cosolvent Resistance.Angewandte Chemie (International ed. in English) · 2021Article
- Machine Learning Enables Selection of Epistatic Enzyme Mutants for Stability Against Unfolding and Detrimental Aggregation.Chembiochem : a European journal of chemical biology · 2021Article
- Computational design of noncanonical amino acid-based thioether staples at N/C-terminal domains of multi-modular pullulanase for thermostabilization in enzyme catalysis.Computational and structural biotechnology journal · 2021Article
- Can constraint network analysis guide the identification phase of KnowVolution? A case study on improved thermostability of an endo-β-glucanase.Computational and structural biotechnology journal · 2021Article
- Aqueous ionic liquids redistribute local enzyme stability via long-range perturbation pathways.Computational and structural biotechnology journal · 2021Article
- iStable 2.0: Predicting protein thermal stability changes by integrating various characteristic modules.Computational and structural biotechnology journal · 2020Article
- Basal Histamine HChemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- Article
- The mechanism of interactions between tea polyphenols and porcine pancreatic alpha-amylase: Analysis by inhibition kinetics, fluorescence quenching, differential scanning calorimetry and isothermal titration calorimetry.Molecular nutrition & food research · 2017Article
- Contribution of single amino acid and codon substitutions to the production and secretion of a lipase by Bacillus subtilis.Microbial cell factories · 2017Article
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3 authors.
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Abstract
Understanding the origin of thermostability is of fundamental importance in protein biochemistry. Opposing views on increased or decreased structural rigidity of the folded state have been put forward in this context. They have been related to differences in the temporal resolution of experiments and computations that probe atomic mobility. Here, we find a significant (p = 0.004) and fair (R2 = 0.46) correlation between the structural rigidity of a well-characterized set of 16 mutants of lipase A from Bacillus subtilis (BsLipA) and their thermodynamic thermostability. We apply the rigidity theory-based Constraint Network Analysis (CNA) approach, analyzing directly and in a time-independent manner the statics of the BsLipA mutants. We carefully validate the CNA results on macroscopic and microscopic experimental observables and probe for their sensitivity with respect to input structures. Furthermore, we introduce a robust, local stability measure for predicting thermodynamic thermostability. Our results complement work that showed for pairs of homologous proteins that raising the structural stability is the most common way to obtain a higher thermostability. Furthermore, they demonstrate that related series of mutants with only a small number of mutations can be successfully analyzed by CNA, which suggests that CNA can be applied prospectively in rational protein design aimed at higher thermodynamic thermostability.
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