ArticlePloS one2015
Transcriptome and Molecular Pathway Analysis of the Hepatopancreas in the Pacific White Shrimp Litopenaeus vannamei under Chronic Low-Salinity Stress.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 1 of them a synthesis that pooled it.
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Who cites it
51 citing papers in PubMed, 1 synthesis or guideline pooled it, 153 citations in OpenAlex.
- Meta-analysis to Unravel Core Transcriptomic Responses in Penaeus vannamei Exposed to Biotic and Abiotic Stresses.Biochemical genetics · 2025Pooled it
- Comparative Transcriptomics Reveals Potential Regulatory Mechanisms Underlying Changes in Antioxidant, Anti-Inflammatory, and Other Immune Functions Induced byAntioxidants (Basel, Switzerland) · 2026Article
- Integrated Analysis of Transcriptome and Metabolome Reveals Molecular Responses to Ammonia Stress in the Gills ofBiology · 2026Article
- Integrative Transcriptomic and Network Analysis of Hemocyte Volume Plasticity and Redox Regulation Under Osmotic Stress inAntioxidants (Basel, Switzerland) · 2026Article
- The functional effects ofFrontiers in physiology · 2026Article
- Transcriptomic Analysis of Litopenaeus vannamei: Understanding Salinity Adaptation Mechanisms in Freshwater Environments.Marine biotechnology (New York, N.Y.) · 2025Article
- Impact of Salinity Stress on Antioxidant Enzyme Activity, Histopathology, and Gene Expression in the Hepatopancreas of the Oriental River Prawn,Animals : an open access journal from MDPI · 2025Article
- Physiological Responses and Histopathological Changes in Narrow-Clawed Crayfish (Animals : an open access journal from MDPI · 2025Article
- Aquatic Organisms in Response to Salinity Stress: Ecological Impacts, Adaptive Mechanisms, and Resilience Strategies.Biology · 2025Review
- Insights into the Regulatory Role of MicroRNAs inBiology · 2025Article
- Effects of Different Salinity Stress on the Transcriptomic Responses of Freshwater Crayfish (Biology · 2024Article
- Effects of Low-Salinity Stress on Histology and Metabolomics in the Intestine ofAnimals : an open access journal from MDPI · 2024Article
- Article
- Transcriptome and molecular regulatory mechanisms analysis of gills in the black tiger shrimpFrontiers in physiology · 2023Article
- Integrative Application of Transcriptomics and Metabolomics Provides Insights into Unsynchronized Growth in Sea Cucumber (International journal of molecular sciences · 2022Article
- The Effect of Salinity Stress on Enzyme Activities, Histology, and Transcriptome of Silver Carp (Biology · 2022Article
- Effects of Low Temperature on Antioxidant and Heat Shock Protein Expression Profiles and Transcriptomic Responses in Crayfish (Antioxidants (Basel, Switzerland) · 2022Article
- Integrated Omics Approaches Revealed the Osmotic Stress-Responsive Genes and Microbiota in Gill of Marine Medaka.mSystems · 2022Article
- Transcriptome Analysis to Study the Molecular Response in the Gill and Hepatopancreas Tissues ofFrontiers in physiology · 2022Article
- A chromosome-level assembly of the black tiger shrimp (Penaeus monodon) genome facilitates the identification of growth-associated genes.Molecular ecology resources · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Pacific white shrimp Litopenaeus vannamei is a euryhaline penaeid species that shows ontogenetic adaptations to salinity, with its larvae inhabiting oceanic environments and postlarvae and juveniles inhabiting estuaries and lagoons. Ontogenetic adaptations to salinity manifest in L. vannamei through strong hyper-osmoregulatory and hypo-osmoregulatory patterns and an ability to tolerate extremely low salinity levels. To understand this adaptive mechanism to salinity stress, RNA-seq was used to compare the transcriptomic response of L. vannamei to changes in salinity from 30 (control) to 3 practical salinity units (psu) for 8 weeks. In total, 26,034 genes were obtained from the hepatopancreas tissue of L. vannamei using the Illumina HiSeq 2000 system, and 855 genes showed significant changes in expression under salinity stress. Eighteen top Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly involved in physiological responses, particularly in lipid metabolism, including fatty-acid biosynthesis, arachidonic acid metabolism and glycosphingolipid and glycosaminoglycan metabolism. Lipids or fatty acids can reduce osmotic stress in L. vannamei by providing additional energy or changing the membrane structure to allow osmoregulation in relevant organs, such as the gills. Steroid hormone biosynthesis and the phosphonate and phosphinate metabolism pathways were also involved in the adaptation of L. vannamei to low salinity, and the differential expression patterns of 20 randomly selected genes were validated by quantitative real-time PCR (qPCR). This study is the first report on the long-term adaptive transcriptomic response of L. vannamei to low salinity, and the results will further our understanding of the mechanisms underlying osmoregulation in euryhaline crustaceans.
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