ArticlePloS one2015
Phytoene Desaturase from Oryza sativa: Oligomeric Assembly, Membrane Association and Preliminary 3D-Analysis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Proteomic analysis of differential responses to norflurazon herbicide in the model green alga Chlamydomonas reinhardtii.Scientific reports · 2025Article
- Combining systems and synthetic biology for in vivo enzymology.The EMBO journal · 2024Article
- Dynamic Changes in Protein-Membrane Association for Regulating Photosynthetic Electron Transport.Cells · 2021Review
- Development and characterization of GR2E Golden rice introgression lines.Scientific reports · 2021Article
- Multiplicity of carotene patterns derives from competition between phytoene desaturase diversification and biological environments.Scientific reports · 2020Article
- Compositional Analysis of Genetically Engineered GR2E "Golden Rice" in Comparison to That of Conventional Rice.Journal of agricultural and food chemistry · 2019Article
- Enzyme-fusion strategies for redirecting and improving carotenoid synthesis inMetabolic engineering communications · 2019Article
- Changing Form and Function through Carotenoids and Synthetic Biology.Plant physiology · 2019Article
- Enzyme Fusion Removes Competition for Geranylgeranyl Diphosphate in Carotenogenesis.Plant physiology · 2019Article
- Limonene dehydrogenase hydroxylates the allylic methyl group of cyclic monoterpenes in the anaerobic terpene degradation byThe Journal of biological chemistry · 2018Article
- AtPDS overexpression in tomato: exposing unique patterns of carotenoid self-regulation and an alternative strategy for the enhancement of fruit carotenoid content.Plant biotechnology journal · 2018Article
- Establishment of an Arabidopsis callus system to study the interrelations of biosynthesis, degradation and accumulation of carotenoids.PloS one · 2018Article
- Article
- The Dual Role of the Plastid Terminal Oxidase PTOX: Between a Protective and a Pro-oxidant Function.Frontiers in plant science · 2015Article
- Characterization of Phytoene Desaturase Knockout Carotenoid-Deficient Microalgal Mutants Generated by Cas9-Ribonucleoprotein Complexes.Physiologia plantarumArticle
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant phytoene desaturase (PDS-His6) from rice was purified to near-homogeneity and shown to be enzymatically active in a biphasic, liposome-based assay system. The protein contains FAD as the sole protein-bound redox-cofactor. Benzoquinones, not replaceable by molecular oxygen, serve as a final electron acceptor defining PDS as a 15-cis-phytoene (donor):plastoquinone oxidoreductase. The herbicidal PDS-inhibitor norflurazon is capable of arresting the reaction by stabilizing the intermediary FAD(red), while an excess of the quinone acceptor relieves this blockage, indicating competition. The enzyme requires its homo-oligomeric association for activity. The sum of data collected through gel permeation chromatography, non-denaturing polyacrylamide electrophoresis, chemical cross-linking, mass spectrometry and electron microscopy techniques indicate that the high-order oligomers formed in solution are the basis for an active preparation. Of these, a tetramer consisting of dimers represents the active unit. This is corroborated by our preliminary X-ray structural analysis that also revealed similarities of the protein fold with the sequence-inhomologous bacterial phytoene desaturase CRTI and other oxidoreductases of the GR2-family of flavoproteins. This points to an evolutionary relatedness of CRTI and PDS yielding different carotene desaturation sequences based on homologous protein folds.
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