ArticlePloS one2015
Comparing Two Intestinal Porcine Epithelial Cell Lines (IPECs): Morphological Differentiation, Function and Metabolism.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 58 citations in OpenAlex.
- Culture Optimization of the IPEC-J2 Piglet Jejunum Cell Line for Applications in Infant Nutrition Research.Molecular nutrition & food research · 2026Article
- Genomic diversity, antibiotic resistance, and maturation‑dependent adhesion of F18 enterotoxigenic Escherichia coli strains in porcine intestinal cells.Gut pathogens · 2026Article
- IPEC-J2 as a cellular model for studying intestinal mucus.Scientific reports · 2025Article
- Iron Stress Reprograms Enterocyte Metabolism.Metabolites · 2025Article
- Discovery and rescue of porcine bastroviruses associated with polioencephalomyelitis in domestic pigs.Journal of virology · 2025Article
- Article
- The proteomic landscape ofFrontiers in cellular and infection microbiology · 2025Article
- Article
- Generation of Porcine and Rainbow Trout 3D Intestinal Models and Their Use to Investigate Astaxanthin Effects In Vitro.International journal of molecular sciences · 2024Article
- Modeling of culture conditions by culture system, glucose and propionic acid and their impact on metabolic profile in IPEC-J2.PloS one · 2024Article
- Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approaches.Materials today. Bio · 2023Article
- Article
- Activity of a Bacteriophage Cocktail to ControlPHAGE (New Rochelle, N.Y.) · 2023Article
- Swine intestinal segment perfusion model for the evaluation of nutrients bioaccessibility.PloS one · 2023Article
- Effect of probiotic bacteria on porcine rotavirus OSU infection of porcine intestinal epithelial IPEC-J2 cells.Archives of virology · 2022Article
- Enhanced Antibacterial Activity of Dermaseptin through Its Immobilization on Alginate Nanoparticles-Effects of Menthol and Lactic Acid on Its Potentialization.Antibiotics (Basel, Switzerland) · 2022Article
- Concerted action of berberine in the porcine intestinal epithelial model IPEC-J2: Effects on tight junctions and apoptosis.Physiological reports · 2022Article
- Porcine and Chicken Intestinal Epithelial Cell Models for Screening Phytogenic Feed Additives-Chances and Limitations in Use as Alternatives to Feeding Trials.Microorganisms · 2022Review
- Seaweed Components as Potential Modulators of the Gut Microbiota.Marine drugs · 2021Review
- A novel method for isolation and culture of primary swine gastric epithelial cells.BMC molecular and cell biology · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pig shows genetical and physiological resemblance to human, which predestines it as an experimental animal model especially for mucosal physiology. Therefore, the intestinal epithelial cell lines 1 and J2 (IPEC-1, IPEC-J2)--spontaneously immortalised cell lines from the porcine intestine--are important tools for studying intestinal function. A microarray (GeneChip Porcine Genome Array) was performed to compare the genome wide gene expression of IPECs. Different significantly up-regulated pathways were identified, like "lysosome", "pathways in cancer", "regulation of actin cytoskeleton" and "oxidative phosphorylation" in IPEC-J2 in comparison to IPEC-1. On the other hand, "spliceosome", "ribosome", "RNA-degradation" and "tight junction" are significantly down-regulated pathways in IPEC-J2 in comparison to IPEC-1. Examined pathways were followed up by functional analyses. ATP-, oxygen, glucose and lactate-measurement provide evidence for up-regulation of oxidative phosphorylation in IPEC-J2. These cells seem to be more active in their metabolism than IPEC-1 cells due to a significant higher ATP-content as well as a higher O2- and glucose-consumption. The down-regulated pathway "ribosome" was followed up by measurement of RNA- and protein content. In summary, IPEC-J2 is a morphologically and functionally more differentiated cell line in comparison to IPEC-1. In addition, IPEC-J2 cells are a preferential tool for in vitro studies with the focus on metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.