ArticleNature2015
Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes.
Article in Nature, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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Who cites it
43 citing papers in PubMed, 87 citations in OpenAlex.
- The Meiotic Spindle, Mechanisms of Chromosome Segregation and Aneuploidy in Aging Human Oocytes: An Update Review.Medicina (Kaunas, Lithuania) · 2026Review
- A novel heterozygous splicing variant in theFrontiers in cell and developmental biology · 2026Article
- Translational repression by 4E-T is crucial to maintain the prophase-I arrest in vertebrate oocytes.Nature communications · 2025Article
- Ovarian Mechanobiology: Understanding the Interplay Between Mechanics and Follicular Development.Cells · 2025Review
- Heterozygous Eif4nif1 Stop-Gain Mice Replicate the Primary Ovarian Insufficiency Phenotype in Women.Endocrinology · 2025Article
- Two mechanisms repress cyclin B1 translation to maintain prophase arrest in mouse oocytes.Nature communications · 2024Article
- A genome-wide perspective of the maternal mRNA translation program during oocyte development.Seminars in cell & developmental biology · 2024Review
- A homozygous stop codon in HORMAD2 in a patient with recurrent digynic triploid miscarriage.Molecular genetics & genomic medicine · 2024Article
- Relationship of quantitative reverse transcription polymerase chain reaction (RT-PCR) to RNA Sequencing (RNAseq) transcriptome identifies mouse preimplantation embryo reference genes†.Biology of reproduction · 2023Article
- Quantitative morphokinetic parameters identify novel dynamics of oocyte meiotic maturation and cumulus expansion†.Biology of reproduction · 2022Article
- Genome-wide quantification of contributions to sexual fitness identifies genes required for spore viability and health in fission yeast.PLoS genetics · 2022Article
- Figla promotes secondary follicle growth in mature mice.Scientific reports · 2021Article
- Caught in the Act: Live-Cell Imaging of Plant Meiosis.Frontiers in plant science · 2021Review
- Maternally contributed Nlrp9b expressed in human and mouse ovarian follicles contributes to early murine preimplantation development.Journal of assisted reproduction and genetics · 2020Article
- 4E-T-bound mRNAs are stored in a silenced and deadenylated form.Genes & development · 2020Article
- The BCL-2 pathway preserves mammalian genome integrity by eliminating recombination-defective oocytes.Nature communications · 2020Article
- FAM46B is a prokaryotic-like cytoplasmic poly(A) polymerase essential in human embryonic stem cells.Nucleic acids research · 2020Article
- The chromatin remodeler Snf2h is essential for oocyte meiotic cell cycle progression.Genes & development · 2020Article
- A novel EIF4ENIF1 mutation associated with a diminished ovarian reserve and premature ovarian insufficiency identified by whole-exome sequencing.Journal of ovarian research · 2019Article
- Inosine RNA modifications are enriched at the codon wobble position in mouse oocytes and eggs†.Biology of reproduction · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
During fertilization, an egg and a sperm fuse to form a new embryo. Eggs develop from oocytes in a process called meiosis. Meiosis in human oocytes is highly error-prone, and defective eggs are the leading cause of pregnancy loss and several genetic disorders such as Down's syndrome. Which genes safeguard accurate progression through meiosis is largely unclear. Here we develop high-content phenotypic screening methods for the systematic identification of mammalian meiotic genes. We targeted 774 genes by RNA interference within follicle-enclosed mouse oocytes to block protein expression from an early stage of oocyte development onwards. We then analysed the function of several genes simultaneously by high-resolution imaging of chromosomes and microtubules in live oocytes and scored each oocyte quantitatively for 50 phenotypes, generating a comprehensive resource of meiotic gene function. The screen generated an unprecedented annotated data set of meiotic progression in 2,241 mammalian oocytes, which allowed us to analyse systematically which defects are linked to abnormal chromosome segregation during meiosis, identifying progression into anaphase with misaligned chromosomes as well as defects in spindle organization as risk factors. This study demonstrates how high-content screens can be performed in oocytes, and allows systematic studies of meiosis in mammals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.