ArticlePloS one2015
Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed.
- Article
- Characterization and development of transcriptome-derived novel EST-SSR markers to assess genetic diversity in3 Biotech · 2023Article
- Unique Salt-Tolerance-Related QTLs, Evolved inPlants (Basel, Switzerland) · 2023Article
- Development and Validation of Gene-Based SSR Markers in the GenusScientifica · 2023Article
- Article
- Association mapping for important agronomic traits in wild and cultivatedFrontiers in genetics · 2022Article
- Genetic Diversity ofFrontiers in plant science · 2022Article
- Progress of Genomics-Driven Approaches for Sustaining Underutilized Legume Crops in the Post-Genomic Era.Frontiers in genetics · 2022Review
- Genic microsatellite marker characterization and development in little millet (Panicum sumatrense) using transcriptome sequencing.Scientific reports · 2021Article
- Transcriptome Analysis Reveals Key Pathways and Candidate Genes Controlling Seed Development and Size in Ricebean (Frontiers in genetics · 2021Article
- Development of EST-SSR markers forPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Article
- Characterization of Drought-Responsive Transcriptome During Seed Germination in Adzuki Bean (Frontiers in genetics · 2020Article
- Transcriptome Sequencing and Development of Novel Genic SSR Markers FromFrontiers in genetics · 2020Article
- Article
- Article
- De novo assembly of transcriptomes, mining, and development of novel EST-SSR markers in Curcuma alismatifolia (Zingiberaceae family) through Illumina sequencing.Scientific reports · 2019Article
- Development of EST-SSR markers and their application in an analysis of the genetic diversity of the endangered species Magnolia sinostellata.Molecular genetics and genomics : MGG · 2019Article
- VigSatDB: genome-wide microsatellite DNA marker database of three species of Vigna for germplasm characterization and improvement.Database : the journal of biological databases and curation · 2019Article
- Comparative genome-wide analysis of WRKY transcription factors in two Asian legume crops: Adzuki bean and Mung bean.Scientific reports · 2018Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The adzuki bean (Vigna angularis (Ohwi) Ohwi and Ohashi) is an important grain legume of Asia. It is cultivated mainly in China, Japan and Korea. Despite its importance, few genomic resources are available for molecular genetic research of adzuki bean. In this study, we developed EST-SSR markers for the adzuki bean through next-generation sequencing. More than 112 million high-quality cDNA sequence reads were obtained from adzuki bean using Illumina paired-end sequencing technology, and the sequences were de novo assembled into 65,950 unigenes. The average length of the unigenes was 1,213 bp. Among the unigenes, 14,547 sequences contained a unique simple sequence repeat (SSR) and 3,350 sequences contained more than one SSR. A total of 7,947 EST-SSRs were identified as potential molecular markers, with mono-nucleotide A/T repeats (99.0%) as the most abundant motif class, followed by AG/CT (68.4%), AAG/CTT (30.0%), AAAG/CTTT (26.2%), AAAAG/CTTTT (16.1%), and AACGGG/CCCGTT (6.0%). A total of 500 SSR markers were randomly selected for validation, of which 296 markers produced reproducible amplicons with 38 polymorphic markers among the 32 adzuki bean genotypes selected from diverse geographical locations across China. The large number of SSR-containing sequences and EST-SSR markers will be valuable for genetic analysis of the adzuki bean and related Vigna species.
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