ArticleNature communications2015
Concentration- and chromosome-organization-dependent regulator unbinding from DNA for transcription regulation in living cells.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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Who cites it
69 citing papers in PubMed.
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- Spatially Resolved Mapping of Voltage-Gated Proton Channel Activity Reveals Delayed Proton Transport in Local Microenvironments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Analysis of Complex Single-Molecule Kinetics: A Protocol for Single-Molecule Interaction Simulations (SMIS).Methods in molecular biology (Clifton, N.J.) · 2026Article
- Conformation-gated binding underlies kinetic asymmetry and negative cooperativity in ATP:cob(I)alamin adenosyltransferase.Cell reports. Physical science · 2025Article
- Transporter excess and clustering facilitate adaptor protein shuttling for bacterial efflux.Cell reports. Physical science · 2025Article
- Transcription factor clusters as information transfer agents.Science advances · 2025Article
- A 'through-DNA' mechanism for co-regulation of metal uptake and efflux.Nature communications · 2024Article
- Article
- Memory effects of transcription regulator-DNA interactions in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Advancements and Practical Considerations for Biophysical Research: Navigating the Challenges and Future of Super-resolution Microscopy.Chemical & biomedical imaging · 2024Review
- Optical sequencing of single synthetic polymers.Nature chemistry · 2024Article
- From Monomers to Hexamers: A Theoretical Probability of the Neighbor Density Approach to Dissect Protein Oligomerization in Cells.Analytical chemistry · 2024Article
- Embracing Heterogeneity: Challenging the Paradigm of Replisomes as Deterministic Machines.Chemical reviews · 2023Review
- Single-cell multimodal imaging uncovers energy conversion pathways in biohybrids.Nature chemistry · 2023Article
- Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy.Chemical & biomedical imaging · 2023Article
- Mechanical stimuli activate gene expression via a cell envelope stress sensing pathway.Scientific reports · 2023Article
- Recognition of Single Fluorescence Events by Temporal Pixel Intensity Fluctuation.Chemical & biomedical imaging · 2023Article
- Probing Oxidant Effects on Superoxide Dismutase 1 Oligomeric States in Live Cells Using Single-Molecule Fluorescence Anisotropy.Chemical & biomedical imaging · 2023Article
- Subcellular redox responses reveal different Cu-dependent antioxidant defenses between mitochondria and cytosol.Metallomics : integrated biometal science · 2022Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Binding and unbinding of transcription regulators at operator sites constitute a primary mechanism for gene regulation. While many cellular factors are known to regulate their binding, little is known on how cells can modulate their unbinding for regulation. Using nanometer-precision single-molecule tracking, we study the unbinding kinetics from DNA of two metal-sensing transcription regulators in living Escherichia coli cells. We find that they show unusual concentration-dependent unbinding kinetics from chromosomal recognition sites in both their apo and holo forms. Unexpectedly, their unbinding kinetics further varies with the extent of chromosome condensation, and more surprisingly, varies in opposite ways for their apo-repressor versus holo-activator forms. These findings suggest likely broadly relevant mechanisms for facile switching between transcription activation and deactivation in vivo and in coordinating transcription regulation of resistance genes with the cell cycle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.