ArticleScientific reports2015
Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- First implementation of a polymer-polymer aqueous two-phase system to improve lateral-flow immunoassay sensitivity via dextranase integration on paper.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Article
- Aqueous Two-Phase Bioinks for Discrete Packing and Compartmentalization of 3D Bioprinted Cells.Advanced healthcare materials · 2026Article
- Advanced Microfluidics for Single Cell-Based Cancer Research.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Cell Partitioning Design for Microfluidic ATPS Devices: A Dynamic Energy Strategy and Calculation Using Chondrocytes and Model Microparticles.Micromachines · 2025Article
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- Aqueous two-phase system (ATPS): from basic science to applications.RSC advances · 2025Review
- Tumoroid-On-a-Plate (ToP): Physiologically Relevant Cancer Model Generation and Therapeutic Screening.Advanced healthcare materials · 2025Article
- Applications and Advances of Multicellular Tumor Spheroids: Challenges in Their Development and Analysis.International journal of molecular sciences · 2023Review
- 3D engineered tissue models for studying human-specific infectious viral diseases.Bioactive materials · 2023Review
- Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines.Cancer & metabolism · 2022Article
- Vascularized Co-Culture Clusteroids of Primary Endothelial and Hep-G2 Cells Based on Aqueous Two-Phase Pickering Emulsions.Bioengineering (Basel, Switzerland) · 2022Article
- Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip.Biosensors · 2021Article
- A High-Throughput Distal Lung Air-Blood Barrier Model Enabled By Density-Driven Underside Epithelium Seeding.Advanced healthcare materials · 2021Article
- Cancer Cell Direct Bioprinting: A Focused Review.Micromachines · 2021Review
- New Born Calf Serum Can Induce Spheroid Formation in Breast Cancer KAIMRC1 Cell Line.Frontiers in molecular biosciences · 2021Article
- Nonspecific characteristics of macromolecules create specific effects in living cells.Biophysical reviews · 2020Review
- Quantitative Size-Based Analysis of Tumor Spheroids and Responses to Therapeutics.Assay and drug development technologies · 2019Article
- Confinement of Suspension-Cultured Cells in Polyethylene Glycol/Polyethylene Oxide-Albumin Aqueous Two-Phase Systems.Frontiers in chemistry · 2019Article
- Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly.Frontiers in chemistry · 2019Article
- Assessing functional connectivity across 3D tissue engineered axonal tracts using calcium fluorescence imaging.Journal of neural engineering · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Various spheroid formation techniques have been widely developed for efficient and reliable 3-D cell culture research. Although those efforts improved many aspects of spheroid generation, the procedures became complex and also required unusual laboratory equipment. Many recent techniques still involve laborious pipetting steps for spheroid manipulation such as collection, distribution and reseeding. In this report, we used a density-controlled polyethylene glycol and dextran aqueous two phase system to generate spheroids that are both consistent in size and precisely size-controllable. Moreover, by adding a few drops of fresh medium to the wells the contain spheroids, they can be simply settled and attached to the culture surface due to reduced densities of the phases. This unique attribute of the technique significantly reduces the numerous pipetting steps of spheroid manipulation to a single pipetting; therefore, the errors from those steps are eliminated and the reliability and efficiency of a research can be maximized.
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