ArticleActa crystallographica. Section F, Structural biology communications2015
Towards time-resolved serial crystallography in a microfluidic device.
Article in Acta crystallographica. Section F, Structural biology communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- BioCARS: Synchrotron facility for probing structural dynamics of biological macromolecules.Structural dynamics (Melville, N.Y.) · 2024Article
- Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.Advanced healthcare materials · 2023Article
- Polymer-based microfluidic device for on-chip counter-diffusive crystallization andLab on a chip · 2023Article
- Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography.Lab on a chip · 2021Article
- A capillary-based microfluidic device enables primary high-throughput room-temperature crystallographic screening.Journal of applied crystallography · 2021Article
- Room-temperature crystallography using a microfluidic protein crystal array device and its application to protein-ligand complex structure analysis.Chemical science · 2020Article
- Mixing and jetting analysis using continuous flow microfluidic sample delivery devices.RSC advances · 2020Article
- The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review.Micromachines · 2020Review
- Protein ensembles link genotype to phenotype.PLoS computational biology · 2019Review
- Sample delivery for serial crystallography at free-electron lasers and synchrotrons.Acta crystallographica. Section D, Structural biology · 2019Review
- Crystal-on-crystal chips for in situ serial diffraction at room temperature.Lab on a chip · 2018Article
- Watching Proteins Function with Time-resolved X-ray Crystallography.Journal of physics D: Applied physics · 2017Article
- Graphene-based microfluidics for serial crystallography.Lab on a chip · 2016Article
- Angular-split/temporal-delay approach to ultrafast protein dynamics at XFELs.Acta crystallographica. Section D, Structural biology · 2016Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Serial methods for crystallography have the potential to enable dynamic structural studies of protein targets that have been resistant to single-crystal strategies. The use of serial data-collection strategies can circumvent challenges associated with radiation damage and repeated reaction initiation. This work utilizes a microfluidic crystallization platform for the serial time-resolved Laue diffraction analysis of macroscopic crystals of photoactive yellow protein (PYP). Reaction initiation was achieved via pulsed laser illumination, and the resultant electron-density difference maps clearly depict the expected pR(1)/pR(E46Q) and pR(2)/pR(CW) states at 10 µs and the pB1 intermediate at 1 ms. The strategies presented here have tremendous potential for extension to chemical triggering methods for reaction initiation and for extension to dynamic, multivariable analyses.
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