ArticleActa crystallographica. Section F, Structural biology communications2015
Do protein crystals nucleate within dense liquid clusters?
Article in Acta crystallographica. Section F, Structural biology communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- A nonclassical pathway of β-hematin crystal nucleation enables its suppression by antimalarials.Communications chemistry · 2025Article
- IntegratedIndustrial & engineering chemistry research · 2025Article
- Resistive Pulse Sensing of Pre-Nucleation Activities during Single-Entity Lysozyme Crystallization on Single Nanopipettes.Sensors and actuators reports · 2025Article
- Mesoscopic p53-rich clusters represent a new class of protein condensates.Biophysics reviews · 2025Review
- An advanced light scattering apparatus for investigating soft matter onboard the International Space Station.NPJ microgravity · 2024Article
- A dual growth mode unique for organic crystals relies on mesoscopic liquid precursors.Communications chemistry · 2024Article
- Exploring the Potential of Nonclassical Crystallization Pathways to Advance Cementitious Materials.Chemical reviews · 2024Review
- Protein nanocondensates: the next frontier.Biophysical reviews · 2023Review
- Rational Design of a Self-Assembling High Performance Organic Nanofluorophore for Intraoperative NIR-II Image-Guided Tumor Resection of Oral Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Topological Considerations in Biomolecular Condensation.Biomolecules · 2023Review
- The pathway from the solution to the steps.Journal of crystal growth · 2022Article
- Supersaturation-Dependent Formation of Amyloid Fibrils.Molecules (Basel, Switzerland) · 2022Review
- Crystal nucleation: Rare made common and captured by Raman.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Accounting for the Presence of Molecular Clusters in Modeling and Interpreting Nucleation and Growth.Crystal growth & design · 2022Article
- Protein conformation and biomolecular condensates.Current research in structural biology · 2022Review
- Mesoscopic protein-rich clusters host the nucleation of mutant p53 amyloid fibrils.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Dynamics of liquid-liquid phase separation of wheat gliadins.Scientific reports · 2018Article
- Recent progress on understanding the mechanisms of amyloid nucleation.Biophysical reviews · 2018Review
- Article
- Nanoscale Dynamics of Protein Assembly Networks in Supersaturated Solutions.Scientific reports · 2017Article
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7 authors.
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Abstract
Protein-dense liquid clusters are regions of high protein concentration that have been observed in solutions of several proteins. The typical cluster size varies from several tens to several hundreds of nanometres and their volume fraction remains below 10(-3) of the solution. According to the two-step mechanism of nucleation, the protein-rich clusters serve as locations for and precursors to the nucleation of protein crystals. While the two-step mechanism explained several unusual features of protein crystal nucleation kinetics, a direct observation of its validity for protein crystals has been lacking. Here, two independent observations of crystal nucleation with the proteins lysozyme and glucose isomerase are discussed. Firstly, the evolutions of the protein-rich clusters and nucleating crystals were characterized simultaneously by dynamic light scattering (DLS) and confocal depolarized dynamic light scattering (cDDLS), respectively. It is demonstrated that protein crystals appear following a significant delay after cluster formation. The cDDLS correlation functions follow a Gaussian decay, indicative of nondiffusive motion. A possible explanation is that the crystals are contained inside large clusters and are driven by the elasticity of the cluster surface. Secondly, depolarized oblique illumination dark-field microscopy reveals the evolution from liquid clusters without crystals to newly nucleated crystals contained in the clusters to grown crystals freely diffusing in the solution. Collectively, the observations indicate that the protein-rich clusters in lysozyme and glucose isomerase solutions are locations for crystal nucleation.
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