ArticleActa crystallographica. Section F, Structural biology communications2015
Trace fluorescent labeling for protein crystallization.
Article in Acta crystallographica. Section F, Structural biology communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Modular Scaffold Crystals for Programmable Installation and Structural Observation of DNA-Binding Proteins.bioRxiv : the preprint server for biology · 2026Article
- Protein crystallization and structure determination at room temperature in the CrystalChip.FEBS open bio · 2025Article
- Ionic Liquids as Protein Crystallization Additives.Crystals · 2021Article
- Protein Crystallization Segmentation and Classification Using Subordinate Color Channel in Fluorescence Microscopy Images.Journal of fluorescence · 2020Article
- Inexpensive robotic system for standard and fluorescent imaging of protein crystals.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Article
- Combining crystallogenesis methods to produce diffraction-quality crystals of a psychrophilic tRNA-maturation enzyme.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Improved protein-crystal identification by using 2,2,2-trichloroethanol as a fluorescence enhancer.Acta crystallographica. Section F, Structural biology communications · 2018Article
- A low-cost method for visible fluorescence imaging.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Feature analysis for classification of trace fluorescent labeled protein crystallization images.BioData mining · 2017Article
- Multivalent Interactions by the Set8 Histone Methyltransferase With Its Nucleosome Substrate.Journal of molecular biology · 2016Article
- Optimizing Associative Experimental Design for Protein Crystallization Screening.IEEE transactions on nanobioscience · 2016Article
- A visible-light-excited fluorescence method for imaging protein crystals without added dyes.Journal of applied crystallography · 2016Article
- Preparation, Crystallization, and Structure Determination of Chromatin Enzyme/Nucleosome Complexes.Methods in enzymology · 2016Article
- CrystPro: Spatiotemporal Analysis of Protein Crystallization Images.Crystal growth & designArticle
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6 authors.
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Abstract
Fluorescence can be a powerful tool to aid in the crystallization of proteins. In the trace-labeling approach, the protein is covalently derivatized with a high-quantum-yield visible-wavelength fluorescent probe. The final probe concentration typically labels ≤0.20% of the protein molecules, which has been shown to not affect the crystal nucleation or diffraction quality. The labeled protein is then used in a plate-screening experiment in the usual manner. As the most densely packed state of the protein is the crystalline form, then crystals show as the brightest objects in the well under fluorescent illumination. A study has been carried out on the effects of trace fluorescent labeling on the screening results obtained compared with nonlabeled protein, and it was found that considering the stochastic nature of the crystal nucleation process the presence of the probe did not affect the outcomes obtained. Other effects are realised when using fluorescence. Crystals are clearly seen even when buried in precipitate. This approach also finds `hidden' leads, in the form of bright spots, with ∼30% of the leads found being optimized to crystals in a single-pass optimization trial. The use of visible fluorescence also enables the selection of colors that bypass interfering substances, and the screening materials do not have to be UV-transparent.
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