Evidence map›Paper›PMID 26143918›Full record

ArticleActa crystallographica. Section D, Biological crystallography2015

Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals.

Justin A Newman, Nicole M Scarborough, Nicholas R Pogranichniy, Rashmi K Shrestha, Richard G Closser, Chittaranjan Das, Garth J Simpson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section D, Biological crystallography, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Depth-of-field extension in optical imaging for rapid crystal screening.Acta crystallographica. Section D, Structural biology · 2021
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Justin A NewmanDepartment of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA.
Nicole M ScarboroughDepartment of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA.
Nicholas R PogranichniyDepartment of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA.
Rashmi K ShresthaDepartment of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA.
Richard G ClosserDepartment of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA.
Chittaranjan DasDepartment of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA.
Garth J SimpsonDepartment of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA.

Funding

Transgenic Mouse Core Facility Shared Resource (TMCF-SR)P30CA023168 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI John Tesmer · 1985 to 2026
$43.4M
Nonlinear Optical Imaging for Guiding Protein Structure DeterminationR01GM103401 · NIGMS · PURDUE UNIVERSITY · PI SIMPSON, GARTH JASON · 2012 to 2018
$1.7M
Ultrafast Nonlinear Optical Approaches toward High-Throughput Membrane Protein NaR01GM103910 · NIGMS · PURDUE UNIVERSITY · PI SIMPSON, GARTH JASON · 2013 to 2016
$1.1M
NCI NIH HHS P30 CA023168NIGMS NIH HHS R01 GM103401NIGMS NIH HHS R01 GM103910NIGMS NIH HHS R01GM-103910
6 · The paper itself

Abstract

The second-harmonic generation (SHG) activity of protein crystals was found to be enhanced by up to ∼1000-fold by the intercalation of SHG phores within the crystal lattice. Unlike the intercalation of fluorophores, the SHG phores produced no significant background SHG from solvated dye or from dye intercalated into amorphous aggregates. The polarization-dependent SHG is consistent with the chromophores adopting the symmetry of the crystal lattice. In addition, the degree of enhancement for different symmetries of dyes is consistent with theoretical predictions based on the molecular nonlinear optical response. Kinetics studies indicate that intercalation arises over a timeframe of several minutes in lysozyme, with detectable enhancements within seconds. These results provide a potential means to increase the overall diversity of protein crystals and crystal sizes amenable to characterization by SHG microscopy.

Indexed as

Aldose-Ketose IsomerasesAnimalsChickensColoring AgentsCrystallizationEndopeptidase KMicroscopyMuramidaseOptical ImagingProteinsRosaniline DyesSchizosaccharomycesSchizosaccharomyces pombe ProteinsAldose-Ketose IsomerasesColoring AgentsEndopeptidase Kmalachite greenMuramidaseProteinsRosaniline DyesSchizosaccharomyces pombe Proteinsxylose isomerasedyehigh-throughput screeningprotein crystalsSHG

Identifiers

PMID26143918
PMCPMC4498603

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.