ArticleThe Journal of general physiology1989
Kinetic time constants independent of previous single-channel activity suggest Markov gating for a large conductance Ca-activated K channel.
Article in The Journal of general physiology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Contradicting Markov.Biophysical journal · 2025Article
- Markov models and long-term memory in ion channels: A contradiction in terms?Biophysical journal · 2025Article
- To what extent naringenin binding and membrane depolarization shape mitoBK channel gating-A machine learning approach.PLoS computational biology · 2022Article
- Moving average filtering with deconvolution (MAD) for hidden Markov model with filtering and correlated noise.European biophysics journal : EBJ · 2019Article
- Mechanosensitivity of the BK Channels in Human Glioblastoma Cells: Kinetics and Dynamical Complexity.The Journal of membrane biology · 2018Article
- Is realistic neuronal modeling realistic?Journal of neurophysiology · 2016Review
- Single-channel kinetics of BK (Slo1) channels.Frontiers in physiology · 2014Review
- Transduction of voltage and Ca2+ signals by Slo1 BK channels.Physiology (Bethesda, Md.) · 2013Review
- Coupling and cooperativity in voltage activation of a limited-state BK channel gating in saturating Ca2+.The Journal of general physiology · 2010Article
- Allosteric control of gating mechanisms revisited: the large conductance Ca2+-activated K+ channel.Biophysical journal · 2009Article
- Linking exponential components to kinetic states in Markov models for single-channel gating.The Journal of general physiology · 2008Article
- Statistical properties of ion channel records. Part II: estimation from the macroscopic current.Mathematical biosciences · 2007Article
- Statistical evaluation of ion-channel gating models based on distributions of log-likelihood ratios.Biophysical journal · 2006Article
- Capsaicin activation of the pain receptor, VR1: multiple open states from both partial and full binding.Biophysical journal · 2003Article
- Gating mechanism of BK (Slo1) channels: so near, yet so far.The Journal of general physiology · 2003Review
- Markovian models of low and high activity levels of cardiac ryanodine receptors.Biophysical journal · 2001Article
- Voltage and Ca2+ activation of single large-conductance Ca2+-activated K+ channels described by a two-tiered allosteric gating mechanism.The Journal of general physiology · 2000Article
- Functional coupling of the beta(1) subunit to the large conductance Ca(2+)-activated K(+) channel in the absence of Ca(2+). Increased Ca(2+) sensitivity from a Ca(2+)-independent mechanism.The Journal of general physiology · 2000Article
- Complex voltage-dependent behavior of single unliganded calcium-sensitive potassium channels.Biophysical journal · 2000Article
- Gating kinetics of single large-conductance Ca2+-activated K+ channels in high Ca2+ suggest a two-tiered allosteric gating mechanism.The Journal of general physiology · 1999Article
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Abstract
Models for the gating of ion channels usually assume that the rate constants for leaving any given kinetic state are independent of previous channel activity. Although such discrete Markov models have been successful in describing channel gating, there is little direct evidence for the Markov assumption of time-invariant rate constants for constant conditions. This paper tests the Markov assumption by determining whether the single-channel kinetics of the large conductance Ca-activated K channel in cultured rat skeletal muscle are independent of previous single-channel activity. The experimental approach is to examine dwell-time distributions conditional on adjacent interval durations. The time constants of the exponential components describing the distributions are found to be independent of adjacent interval duration, and hence, previous channel activity. In contrast, the areas of the different components can change. Since the observed time constants are a function of the underlying rate constants for transitions among the kinetic states, the observation of time constants independent of previous channel activity suggests that the rate constants are also independent of previous channel activity. Thus, the channel kinetics are consistent with Markov gating. An observed dependent (inverse) relationship between durations of adjacent open and shut intervals together with Markov gating indicates that there are two or more independent transition pathways connecting open and shut states. Finally, no evidence is found to suggest that gating is not at thermodynamic equilibrium: the inverse relationship was independent of the time direction of analysis.
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