Evidence map›Paper›PMID 26143376›Full record

ArticleFEBS letters2015

In vitro substrate specificities of 3'-5' polymerases correlate with biological outcomes of tRNA 5'-editing reactions.

Yicheng Long, Jane E Jackman

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Yicheng LongDepartment of Chemistry and Biochemistry, Center for RNA Biology and Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, United States.
Jane E JackmanDepartment of Chemistry and Biochemistry, Center for RNA Biology and Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, United States. Electronic address: jackman.14@osu.edu.

Funding

Molecular Mechanism and Biological Function of 3'-5' PolymerasesR01GM087543 · NIGMS · OHIO STATE UNIVERSITY · PI JACKMAN, JANE ELIZABETH · 2010 to 2019
$2.7M
NIGMS NIH HHS GM087543NIGMS NIH HHS R01 GM087543
6 · The paper itself

Abstract

Protozoan mitochondrial tRNAs (mt-tRNAs) are repaired by a process known as 5'-editing. Mt-tRNA sequencing revealed organism-specific patterns of editing G-U base pairs, wherein some species remove G-U base pairs during 5'-editing, while others retain G-U pairs in the edited tRNA. We tested whether 3'-5' polymerases that catalyze the repair step of 5'-editing exhibit organism-specific preferences that explain the treatment of G-U base pairs. Biochemical and kinetic approaches revealed that a 3'-5' polymerase from Acanthamoeba castellanii tolerates G-U wobble pairs in editing substrates much more readily than several other enzymes, consistent with its biological pattern of editing.

Indexed as

RNA EditingAcanthamoeba castellaniiDictyosteliumElectrophoresis, Polyacrylamide GelIsoenzymesIsotope LabelingKineticsMitochondriaModels, MolecularPhosphorus RadioisotopesProtozoan ProteinsRecombinant ProteinsRNA NucleotidyltransferasesRNA, ProtozoanRNA, TransferRNA, Transfer, IleIsoenzymesPhosphorus RadioisotopesProtozoan ProteinsRecombinant ProteinsRNA NucleotidyltransferasesRNA, ProtozoanRNA, TransferRNA, Transfer, IletRNA nucleotidyltransferase3′–5′ polymeraseAcanthamoeba castellaniiDictyostelium discoideumMitochondrial tRNAThg1-like proteintRNA editing

Identifiers

PMID26143376
PMCPMC4524524

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.