ArticleTheriogenology2015
Pig oocyte activation using a Zn²⁺ chelator, TPEN.
Article in Theriogenology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Endometriosis disrupts oocyte metabolism and cortical granule function, impairing fertilization.Reproductive biology and endocrinology : RB&E · 2026Article
- A comprehensive review about sperm-oocyte interactions and key activation factors of fertilization in mammals.The Canadian journal of urology · 2026Review
- Factors Influencing the Production Efficiency of Cloned Pigs: A Large-Scale Retrospective Analysis.Animals : an open access journal from MDPI · 2026Article
- Molecular Mechanism of Oocyte Activation in Mammals: Past, Present, and Future Directions.Biomolecules · 2024Review
- Calcium signaling in oocyte quality and functionality and its application.Frontiers in endocrinology · 2024Review
- - Invited Review - Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm.Animal bioscience · 2023Article
- Applications of advances in mRNA-based platforms as therapeutics and diagnostics in reproductive technologies.Frontiers in cell and developmental biology · 2023Review
- Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation.Animals : an open access journal from MDPI · 2022Article
- Molecular Characterization and the Essential Biological Function of the Metal Chaperone Protein MtmA in Aspergillus fumigatus.Applied and environmental microbiology · 2022Article
- Recurrent spontaneous oocyte activation causes female infertility.Journal of assisted reproduction and genetics · 2022Article
- Oocyte activation deficiency and assisted oocyte activation: mechanisms, obstacles and prospects for clinical application.Human reproduction open · 2022Review
- Review
- Role of zinc in female reproduction.Biology of reproduction · 2021Review
- A novel swine sex-linked marker and its application across different mammalian species.Transgenic research · 2020Article
- Perfect date-the review of current research into molecular bases of mammalian fertilization.Journal of assisted reproduction and genetics · 2020Review
- Bovine eggs release zinc in response to parthenogenetic and sperm-induced egg activation.Theriogenology · 2019Article
- Zinc sparks induce physiochemical changes in the egg zona pellucida that prevent polyspermy.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- The zinc spark is an inorganic signature of human egg activation.Scientific reports · 2016Article
- The fertilization-induced zinc spark is a novel biomarker of mouse embryo quality and early development.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Artificial oocyte activation is a critical step during SCNT. Most current activation protocols focus on inducing an increase in the intracellular free Ca(2+) concentration of the oocyte. Here, we have used a zinc chelator, TPEN, to enhance the efficiency of oocyte activation during SCNT. TPEN treatment of matured pig oocytes resulted in the reduction of available Zn(2+) in pig oocytes; however, the cytosolic Ca(2+) concentration in the oocytes was not affected by the TPEN treatment. When various concentrations (100-250 μM) and incubation durations (45 minutes-2.5 hours) of TPEN were used to activate oocytes, the efficiency of oocyte activation was not different from conventional activation methods. When oocytes that were activated by conventional activation methods were incubated with a lower concentration of TPEN (5-10 μM), a significant increase in embryos developing to the blastocyst stage was observed. In addition, when oocytes receiving a small Ca(2+) stimulus were further activated by higher concentration of TPEN (100-200 μM), a significant increase in the frequency of blastocyst formation was observed, compared to a conventional activation method. This result indicated that TPEN can be a main reagent in oocyte activation. No increase in the cytosolic Ca(2+) level was detected when oocytes were exposed to various concentrations of TPEN, indicating the ability of TPEN to induce oocyte activation is independent of an intracellular Ca(2+) increase. We were able to produce clones through SCNT by using the TPEN-assisted activation procedure, and the piglets produced through the process did not show any signs of abnormality. In this study, we have developed an efficient way to use TPEN to increase the developmental potential of cloned embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.