ReviewCurrent opinion in chemical biology2015
The evolving capabilities of rhodopsin-based genetically encoded voltage indicators.
Review in Current opinion in chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
15 citing papers in PubMed.
- QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins.Nature communications · 2022Article
- Advancements in the Quest to Map, Monitor, and Manipulate Neural Circuitry.Frontiers in neural circuits · 2022Review
- Near-infrared and far-red genetically encoded indicators of neuronal activity.Journal of neuroscience methods · 2021Article
- High-speed compressed-sensing fluorescence lifetime imaging microscopy of live cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor Archon2.International journal of molecular sciences · 2020Article
- Photocycle Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1.International journal of molecular sciences · 2019Article
- Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1.International journal of molecular sciences · 2019Article
- Multiphoton Intravital Calcium Imaging.Current protocols in cytometry · 2018Article
- Multi-scale approaches for high-speed imaging and analysis of large neural populations.PLoS computational biology · 2017Article
- A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging.Annual review of physiology · 2017Review
- Illuminating Brain Activities with Fluorescent Protein-Based Biosensors.Chemosensors (Basel, Switzerland) · 2017Article
- Advances in two photon scanning and scanless microscopy technologies for functional neural circuit imaging.Proceedings of the IEEE. Institute of Electrical and Electronics Engineers · 2017Article
- Advances in Intravital Non-Linear Optical Imaging of the Central Nervous System in Rodents.Brain plasticity (Amsterdam, Netherlands) · 2016Review
- Genetically Encoded Voltage Indicators: Opportunities and Challenges.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Review
- Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters.Biophysical reviews · 2016Review
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
Protein engineering over the past four years has made rhodopsin-based genetically encoded voltage indicators a leading candidate to achieve the task of reporting action potentials from a population of genetically targeted neurons in vivo. Rational design and large-scale screening efforts have steadily improved the dynamic range and kinetics of the rhodopsin voltage-sensing domain, and coupling these rhodopsins to bright fluorescent proteins has supported bright fluorescence readout of the large and rapid rhodopsin voltage response. The rhodopsin-fluorescent protein fusions have the highest achieved signal-to-noise ratios for detecting action potentials in neuronal cultures to date, and have successfully reported single spike events in vivo. Given the rapid pace of current development, the genetically encoded voltage indicator class is nearing the goal of robust spike imaging during live-animal behavioral experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.