Evidence map›Paper›PMID 26143085›Full record

ArticleProstaglandins, leukotrienes, and essential fatty acids2015

A high fat diet containing saturated but not unsaturated fatty acids enhances T cell receptor clustering on the nanoscale.

Saame Raza Shaikh, Sarah Boyle, Michael Edidin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Prostaglandins, leukotrienes, and essential fatty acids, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Regulation of monoamine transporters and receptors by lipid microdomains: implications for depression.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018
    Review
  2. Harnessing the Induction of CD8Frontiers in immunology · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Saame Raza ShaikhDepartment of Biochemistry & Molecular Biology, East Carolina Diabetes & Obesity Institute, East Carolina University, 600 Moye Blvd, Greenville, NC 27834, USA. Electronic address: shaikhsa@ecu.edu.
Sarah BoyleDepartment of Biology, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.
Michael EdidinDepartment of Biology, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.
Johns Hopkins University · USEast Carolina University · US

Funding

The influence of TCR spatial organization on the T cell responsesP01AI072677 · NIAID · JOHNS HOPKINS UNIVERSITY · PI SCHNECK, JONATHAN P · 2008 to 2012
$10.3M
Suppressing inflammation and boosting humoral immunity with n-3 PUFAsR01AT008375 · NCCIH · UNIV OF NORTH CAROLINA CHAPEL HILL · PI SHAIKH, SAAME R · 2015 to 2019
$1.9M
NCCIH NIH HHS R01 AT008375NCCIH NIH HHS R01AT008375NIAID NIH HHS 1P01AI072677NIAID NIH HHS P01 AI072677
6 · The paper itself

Abstract

Cell culture studies show that the nanoscale lateral organization of surface receptors, their clustering or dispersion, can be altered by changing the lipid composition of the membrane bilayer. However, little is known about similar changes in vivo, which can be effected by changing dietary lipids. We describe the use of a newly developed method, k-space image correlation spectroscopy, kICS, for analysis of quantum dot fluorescence to show that a high fat diet can alter the nanometer-scale clustering of the murine T cell receptor, TCR, on the surface of naive CD4(+) T cells. We found that diets enriched primarily in saturated fatty acids increased TCR nanoscale clustering to a level usually seen only on activated cells. Diets enriched in monounsaturated or n-3 polyunsaturated fatty acids had no effect on TCR clustering. Also none of the high fat diets affected TCR clustering on the micrometer scale. Furthermore, the effect of the diets was similar in young and middle aged mice. Our data establish proof-of-principle that TCR nanoscale clustering is sensitive to the composition of dietary fat.

Indexed as

Diet, High-FatAnimalsCD4-Positive T-LymphocytesFatty AcidsFatty Acids, MonounsaturatedFatty Acids, Omega-3MiceMice, TransgenicProtein MultimerizationReceptors, Antigen, T-CellSpectrum AnalysisFatty AcidsFatty Acids, MonounsaturatedFatty Acids, Omega-3Receptors, Antigen, T-CellFatty acidsFluorescenceHigh fat dietQuantum dotsT cell receptor

Identifiers

PMID26143085
PMCPMC4554807
OpenAlexW1766560169

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.