ArticleAnalytical and bioanalytical chemistry2015
Ultrasensitive microfluidic array for serum pro-inflammatory cytokines and C-reactive protein to assess oral mucositis risk in cancer patients.
Article in Analytical and bioanalytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Biomarkers in the assessment of oral mucositis in head and neck cancer patients: a systematic review and meta-analysis.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2017Pooled it
- Pretreatment C-Reactive Protein/Albumin Ratio Can Predict Severity of Radiotherapy-Induced Oral Mucositis in Advanced Laryngeal Cancer Patients.Oral diseases · 2026Article
- Bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays in clinical diagnosis and treatment.Mechanobiology in medicine · 2024Review
- Current advances in noninvasive methods for the diagnosis of oral squamous cell carcinoma: a review.European journal of medical research · 2023Review
- Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases.Sensors (Basel, Switzerland) · 2020Review
- Review
- Comparison of Antibacterial Effect of Cationic Peptide LL-37 and Cefalexin on Clinical Staphylococcus aureus-induced Infection after Femur Fracture Fixation.Orthopaedic surgery · 2020Article
- Article
- Accessible Telemedicine Diagnostics with ELISA in a 3D Printed Pipette Tip.Analytical chemistry · 2019Article
- Bioassay for monitoring the anti-aging effect of cord blood treatment.Theranostics · 2019Article
- Article
- Automated 3D-Printed Microfluidic Array for Rapid Nanomaterial-Enhanced Detection of Multiple Proteins.Analytical chemistry · 2018Article
- Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection.Sensors (Basel, Switzerland) · 2018Article
- Sliding-strip microfluidic device enables ELISA on paper.Biosensors & bioelectronics · 2018Article
- Effects ofTheScientificWorldJournal · 2018Review
- Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy treatment for head and neck squamous cell carcinoma.Clinical oral investigations · 2017Article
- Recent Progress in Nanomaterial-Based Electrochemical Biosensors for Cancer Biomarkers: A Review.Molecules (Basel, Switzerland) · 2017Review
- Multiplexed Electrochemical Immunosensors for Clinical Biomarkers.Sensors (Basel, Switzerland) · 2017Review
- Emerging Cytokine Biosensors with Optical Detection Modalities and Nanomaterial-Enabled Signal Enhancement.Sensors (Basel, Switzerland) · 2017Review
- Automated 3D-printed unibody immunoarray for chemiluminescence detection of cancer biomarker proteins.Lab on a chip · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
In addition to disease diagnostics, there is a need for biomarkers to predict severity of cancer therapy side effects such as oral mucositis. Oral mucositis is an inflammatory lesion of oral mucosa caused by high-dose chemotherapy and/or radiation that is especially prevalent during oral cancer treatment. We describe here a semi-automated, modular microfluidic immunoarray optimized for ultrasensitive detection of pro-inflammatory cytokines involved in pathobiology of oral mucositis. Our goal is to methodologically identify risk of mucositis early in oral cancer treatment, before the onset of lesions. Biomarkers include tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and C-reactive protein (CRP). Protein analytes were captured from serum in a capture chamber by 1-μm magnetic beads coated with antibodies and enzyme labels. Beads are then transported downstream to a detection chamber containing an eight-sensor array coated with glutathione-coated gold nanoparticles (GSH-AuNP) and a second set of antibodies to capture the beads with analyte proteins. In this first application of the immunoarray to four-protein multiplexed measurements, ultralow detection limits of 10-40 fg mL(-1) in 5 μL serum were achieved for simultaneous detection in 30 min. Mass detection limits were 2.5-10 zmol, as few as 1500 molecules. Accuracy and diagnostic utility of the arrays were demonstrated by correlation of levels of the four biomarker proteins in serum from head and neck cancer patients with results from standard ELISA. This approach may lead to rapid, low-cost estimates of projected risk for severity of oral mucositis in cancer patients to enable improved therapeutic management.
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Registered trials
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