Evidence map›Paper›PMID 26142780›Full record

ArticleBiomaterials2015

Biodegradable nano-films for capture and non-invasive release of circulating tumor cells.

Wei Li, Eduardo Reátegui, Myoung-Hwan Park, Steven Castleberry, Jason Z Deng, Bryan Hsu, Sarah Mayner, Anne E Jensen, Lecia V Sequist, Shyamala Maheswaran and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. Review
  2. Rapid isolation and recovery ofMaterials today. Bio · 2025
    Article
  3. Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Review
  9. Capture and Selective Release of Viable Circulating Tumor Cells.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  10. Review
  11. Review
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Wei Li *Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Eduardo Reátegui *Department of Surgery, Harvard Medical School, Boston, MA, USA.
Myoung-Hwan ParkDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Steven CastleberryDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Jason Z DengDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Bryan HsuDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Sarah MaynerDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Anne E JensenCenter for Engineering in Medicine, Harvard Medical School, Boston, MA, USA.
Lecia V SequistMassachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA, USA.
Shyamala MaheswaranDepartment of Surgery, Harvard Medical School, Boston, MA, USA.
Daniel A HaberMassachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA, USA.
Mehmet TonerDepartment of Surgery, Harvard Medical School, Boston, MA, USA.
Shannon L StottCenter for Engineering in Medicine, Harvard Medical School, Boston, MA, USA.
Paula T HammondDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Funding

Point-of care Microfluidics for Early Detection of CancerU01EB012493 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI HABER, DANIEL A., TONER, MEHMET · 2010 to 2018
$19.6M
Bio-MicroElectroMechanical Systems Resource CenterP41EB002503 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI TONER, MEHMET · 2004 to 2018
$17.4M
Point-of-Care Microfluidics in Lung CancerR01EB008047 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI TONER, MEHMET · 2007 to 2009
$3.4M
NIBIB NIH HHS EB008047NIBIB NIH HHS P41 EB002503NIBIB NIH HHS P41 EB002503-11NIBIB NIH HHS R01 EB008047NIBIB NIH HHS U01 EB012493
6 · The paper itself

Abstract

Selective isolation and purification of circulating tumor cells (CTCs) from whole blood is an important capability for both clinical medicine and biological research. Current techniques to perform this task place the isolated cells under excessive stresses that reduce cell viability, and potentially induce phenotype change, therefore losing valuable information about the isolated cells. We present a biodegradable nano-film coating on the surface of a microfluidic chip, which can be used to effectively capture as well as non-invasively release cancer cell lines such as PC-3, LNCaP, DU 145, H1650 and H1975. We have applied layer-by-layer (LbL) assembly to create a library of ultrathin coatings using a broad range of materials through complementary interactions. By developing an LbL nano-film coating with an affinity-based cell-capture surface that is capable of selectively isolating cancer cells from whole blood, and that can be rapidly degraded on command, we are able to gently isolate cancer cells and recover them without compromising cell viability or proliferative potential. Our approach has the capability to overcome practical hurdles and provide viable cancer cells for downstream analyses, such as live cell imaging, single cell genomics, and in vitro cell culture of recovered cells. Furthermore, CTCs from cancer patients were also captured, identified, and successfully released using the LbL-modified microchips.

Indexed as

AlginatesBiocompatible MaterialsCell CountCell Line, TumorCell SeparationGlucuronic AcidHexuronic AcidsHumansMicrofluidic Analytical TechniquesNanostructuresNeoplasmsNeoplastic Cells, CirculatingPolyaminesSurface PropertiesAlginatesBiocompatible MaterialsGlucuronic AcidHexuronic AcidspolyallylaminePolyaminesAlginateBiodegradableCirculating tumor cellsLayer-by-layerNano-filmSurface modification

Identifiers

PMID26142780
PMCPMC4827098

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.