ArticlePflugers Archiv : European journal of physiology2016
Functional response of the isolated, perfused normoxic heart to pyruvate dehydrogenase activation by dichloroacetate and pyruvate.
Article in Pflugers Archiv : European journal of physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Redox coupling of lactate and β-hydroxybutyrate: An inter-organ circuit linking metabolic flexibility, mitochondrial adaptation, and disease.Redox biology · 2026Review
- Mitochondrial transplantation for cardioprotection and induction of angiogenesis in ischemic heart disease.Stem cell research & therapy · 2025Review
- Post-translational modifications of pyruvate dehydrogenase complex in cardiovascular disease.iScience · 2024Review
- Dichloroacetate ameliorates myocardial ischemia-reperfusion injury via regulating autophagy and glucose homeostasis.Archives of medical science : AMS · 2023Article
- Synchronizing systolic calcium release with azumolene in an experimental model.Heart rhythm O2 · 2022Article
- Article
- Pyruvate as a Potential Beneficial Anion in Resuscitation Fluids.Frontiers in medicine · 2022Article
- Dihydrolipoamide dehydrogenase, pyruvate oxidation, and acetylation-dependent mechanisms intersecting drug iatrogenesis.Cellular and molecular life sciences : CMLS · 2021Review
- Cardioprotective effects of dantrolene in doxorubicin-induced cardiomyopathy in mice.Heart rhythm O2 · 2021Article
- KairoSight: Open-Source Software for the Analysis of Cardiac Optical Data Collected From Multiple Species.Frontiers in physiology · 2021Article
- Potential Consequences of the Red Blood Cell Storage Lesion on Cardiac Electrophysiology.Journal of the American Heart Association · 2020Article
- Activation of Oxytocin Neurons Improves Cardiac Function in a Pressure-Overload Model of Heart Failure.JACC. Basic to translational science · 2020Article
- Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of DiabeticJournal of diabetes research · 2020Article
- Plasticizer Interaction With the Heart: Chemicals Used in Plastic Medical Devices Can Interfere With Cardiac Electrophysiology.Circulation. Arrhythmia and electrophysiology · 2019Article
- Article
- ElectroMap: High-throughput open-source software for analysis and mapping of cardiac electrophysiology.Scientific reports · 2019Article
- Lights, camera, path splitter: a new approach for truly simultaneous dual optical mapping of the heart with a single camera.BMC biomedical engineering · 2019Article
- Propionate-induced changes in cardiac metabolism, notably CoA trapping, are not altered by l-carnitine.American journal of physiology. Endocrinology and metabolism · 2018Article
- Disruption of neonatal cardiomyocyte physiology following exposure to bisphenol-a.Scientific reports · 2018Article
- Pyruvate is a prospective alkalizer to correct hypoxic lactic acidosis.Military Medical Research · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Dichloroacetate (DCA) and pyruvate activate pyruvate dehydrogenase (PDH), a key enzyme that modulates glucose oxidation and mitochondrial NADH production. Both compounds improve recovery after ischemia in isolated hearts. However, the action of DCA and pyruvate in normoxic myocardium is incompletely understood. We measured the effect of DCA and pyruvate on contraction, mitochondrial redox state, and intracellular calcium cycling in isolated rat hearts during normoxic perfusion. Normalized epicardial NADH fluorescence (nNADH) and left ventricular developed pressure (LVDP) were measured before and after administering DCA (5 mM) or pyruvate (5 mM). Optical mapping of Rhod-2AM was used to measure cytosolic calcium kinetics. DCA maximally activated PDH, increasing the ratio of active to total PDH from 0.48 ± 0.03 to 1.03 ± 0.03. Pyruvate sub-maximally activated PDH to a ratio of 0.75 ± 0.02. DCA and pyruvate increased LVDP. When glucose was the only exogenous fuel, pyruvate increased nNADH by 21.4 ± 2.9 % while DCA reduced nNADH by 21.4 ± 6.1 % and elevated the incidence of premature ventricular contractions (PVCs). When lactate, pyruvate, and glucose were provided together as exogenous fuels, nNADH increased with DCA, indicating that PDH activation with glucose as the only exogenous fuel depletes PDH substrate. Calcium transient time-to-peak was shortened by DCA and pyruvate and SR calcium re-uptake was 30 % longer. DCA and pyruvate increased SR calcium load in myocyte monolayers. Overall, during normoxia when glucose is the only exogenous fuel, DCA elevates SR calcium, increases LVDP and contractility, and diminishes mitochondrial NADH. Administering DCA with plasma levels of lactate and pyruvate mitigates the drop in mitochondrial NADH and prevents PVCs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.