ArticleEMBO reports2015
Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages.
Article in EMBO reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Substrates mimicking the blastocyst geometry revert pluripotent stem cell to naivety.Nature materials · 2024Article
- Delayed puberty, gonadotropin abnormalities and subfertility in male Padi2/Padi4 double knockout mice.Reproductive biology and endocrinology : RB&E · 2022Article
- Quantitative cellular-resolution map of the oxytocin receptor in postnatally developing mouse brains.Nature communications · 2020Article
- The transition from local to global patterns governs the differentiation of mouse blastocysts.PloS one · 2020Article
- Lineage marker synchrony in hematopoietic genealogies refutes the PU.1/GATA1 toggle switch paradigm.Nature communications · 2018Article
- Cardiomyocytes of the Heart and Pulmonary Veins: Novel Contributors to Asthma?American journal of respiratory cell and molecular biology · 2017Review
- The RNA mMolecular cell · 2017Article
- Satellite-like cells contribute to pax7-dependent skeletal muscle repair in adult zebrafish.Developmental biology · 2017Article
- The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo.Developmental cell · 2017Article
- Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.Scientific reports · 2016Article
- Inverted light-sheet microscope for imaging mouse pre-implantation development.Nature methods · 2016Article
- αT-catenin in restricted brain cell types and its potential connection to autism.Journal of molecular psychiatry · 2016Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian development begins with the segregation of embryonic and extra-embryonic lineages in the blastocyst. Recent studies revealed cell-to-cell gene expression heterogeneity and dynamic cell rearrangements during mouse blastocyst formation. Thus, mechanistic understanding of lineage specification requires quantitative description of gene expression dynamics at a single-cell resolution in living embryos. However, only a few fluorescent gene expression reporter mice are available and quantitative live image analysis is limited so far. Here, we carried out a fluorescence gene-trap screen and established reporter mice expressing Venus specifically in the first lineages. Lineage tracking, quantitative gene expression and cell position analyses allowed us to build a comprehensive lineage map of mouse pre-implantation development. Our systematic analysis revealed that, contrary to the available models, the timing and mechanism of lineage specification may be distinct between the trophectoderm and the inner cell mass. While expression of our trophectoderm-specific lineage marker is upregulated in outside cells upon asymmetric divisions at 8- and 16-cell stages, the inside-specific upregulation of the inner-cell-mass marker only becomes evident at the 64-cell stage. This study thus provides a framework toward systems-level understanding of embryogenesis marked by high dynamicity and stochastic variability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.