ArticleMetabolic engineering2015
Precise metabolic engineering of carotenoid biosynthesis in Escherichia coli towards a low-cost biosensor.
Article in Metabolic engineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Transcription factor-based biosensors for screening and dynamic regulation.Frontiers in bioengineering and biotechnology · 2023Review
- Strategies for Improving Small-Molecule Biosensors in Bacteria.Biosensors · 2022Review
- Gut Microbes and Eye Disease.Ophthalmic research · 2022Review
- Dynamic Control of Gene Expression with Riboregulated Switchable Feedback Promoters.ACS synthetic biology · 2021Article
- Improvement of l-Valine Production by Atmospheric and Room Temperature Plasma Mutagenesis and High-Throughput Screening inACS omega · 2020Article
- Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences.Frontiers in microbiology · 2020Review
- Dynamic and tunable metabolite control for robust minimal-equipment assessment of serum zinc.Nature communications · 2019Article
- Point-of-care biomarker quantification enabled by sample-specific calibration.Science advances · 2019Article
- In vivo biosensors: mechanisms, development, and applications.Journal of industrial microbiology & biotechnology · 2018Article
- Small molecule signaling, regulation, and potential applications in cellular therapeutics.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- Development of a Pigment-Based Whole-Cell Zinc Biosensor for Human Serum.ACS synthetic biology · 2018Article
- Metabolomics Analysis of the Toxic Effects of the Production of Lycopene and Its Precursors.Frontiers in microbiology · 2018Article
- Precise control of lycopene production to enable a fast-responding, minimal-equipment biosensor.Metabolic engineering · 2017Article
- Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems.Metabolic engineering · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Micronutrient deficiencies, including zinc deficiency, are responsible for hundreds of thousands of deaths annually. A key obstacle to allocating scarce treatment resources is the ability to measure population blood micronutrient status inexpensively and quickly enough to identify those who most need treatment. This paper develops a metabolically engineered strain of Escherichia coli to produce different colored pigments (violacein, lycopene, and β-carotene) in response to different extracellular zinc levels, for eventual use in an inexpensive blood zinc diagnostic test. However, obtaining discrete color states in the carotenoid pathway required precise engineering of metabolism to prevent reaction at low zinc concentrations but allow complete reaction at higher concentrations, and all under the constraints of natural regulator limitations. Hence, the metabolic engineering challenge was not to improve titer, but to enable precise control of pathway state. A combination of gene dosage, post-transcriptional, and post-translational regulation was necessary to allow visible color change over physiologically relevant ranges representing a small fraction of the regulator's dynamic response range, with further tuning possible by modulation of precursor availability. As metabolic engineering expands its applications and develops more complex systems, tight control of system components will likely become increasingly necessary, and the approach presented here can be generalized to other natural sensing systems for precise control of pathway state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.