ArticleCell2015
Quantifying Protein-mRNA Interactions in Single Live Cells.
Article in Cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 93 citations in OpenAlex.
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- Post-transcriptional regulatory networks: The dynamic interplay of RNA-binding proteins.The FEBS journal · 2026Review
- Single-protein/RNA imaging reveals ZNF598 as a limiting factor in resolving collided ribosomes.The EMBO journal · 2025Article
- Allosteric genetically encoded biosensor for spatiotemporal monitoring of endogenous RNA dynamics in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- IGF2BP1 phosphorylation in the disordered linkers regulates ribonucleoprotein condensate formation and RNA metabolism.Nature communications · 2024Article
- Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.Developmental cell · 2024Article
- m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation.Nature communications · 2024Article
- Real-time single-molecule imaging of transcriptional regulatory networks in living cells.Nature reviews. Genetics · 2024Review
- Is there a localized role for translational quality control?RNA (New York, N.Y.) · 2023Article
- Direct m6A recognition by IMP1 underlays an alternative model of target selection for non-canonical methyl-readers.Nucleic acids research · 2023Article
- Tracking the Message: Applying Single Molecule Localization Microscopy to Cellular RNA Imaging.Chembiochem : a European journal of chemical biology · 2023Review
- Cap-dependent translation initiation monitored in living cells.Nature communications · 2022Article
- Tagged actin mRNA dysregulation in IGF2BP1[Formula: see text] mice.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Probing Notch1-Dll4 signaling in regulating osteogenic differentiation of human mesenchymal stem cells using single cell nanobiosensor.Scientific reports · 2022Article
- Illuminating RNA biology through imaging.Nature cell biology · 2022Review
- A Ribosomal Perspective on Neuronal Local Protein Synthesis.Frontiers in molecular neuroscience · 2022Review
- RNA and neuronal function: the importance of post-transcriptional regulation.Oxford open neuroscience · 2022Review
- Imaging Organization of RNA Processing within the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- The translatome of neuronal cell bodies, dendrites, and axons.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- RNA transport from transcription to localized translation: a single molecule perspective.RNA biology · 2021Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Specific binding proteins are crucial for the correct spatiotemporal expression of mRNA. To understand this process, a method is required to characterize RNA-protein interactions in single living cells with subcellular resolution. We combined endogenous single RNA and protein detection with two-photon fluorescence fluctuation analysis to measure the average number of proteins bound to mRNA at specific locations within live cells. We applied this to quantify the known binding of zipcode binding protein 1 (ZBP1) and ribosomes to β-actin mRNA within subcellular compartments of primary fibroblasts and neurons. ZBP1-mRNA binding did not occur in nuclei, contrary to previous conclusions. ZBP1 interaction with β-actin mRNA was enhanced perinuclearly in neurons compared to fibroblasts. Cytoplasmic ZBP1 and ribosome binding to the mRNA were anti-correlated depending on their location in the cell. These measurements support a mechanism whereby ZBP1 inhibits translation of localizing mRNA until its release from the mRNA peripherally, allowing ribosome binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.