ArticleInternational journal of molecular sciences2015
Decellularized Human Skeletal Muscle as Biologic Scaffold for Reconstructive Surgery.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
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Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it, 113 citations in OpenAlex.
- Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives.International journal of molecular sciences · 2018Pooled it
- In vivo evaluation of decellularized skeletal muscle matrices for skeletal muscle repair: A systematic review.Bioengineering & translational medicine · 2025Review
- Tensile Forces and Nanofiber Alignment Influence Both Innervated and Non-Innervated Skeletal Myofiber Formation in Custom Mechanobioreactors.Biotechnology journal · 2025Article
- Controlled delivery of PRP from decellularized extracellular matrix enhances skeletal muscle regeneration.Scientific reports · 2025Article
- Adipose Decellularized Matrix: A Promising Skeletal Muscle Tissue Engineering Material for Volume Muscle Loss.Biomaterials research · 2025Review
- A sterilization method for human decellularized vaginal matrices.Scientific reports · 2024Article
- Comparison of skeletal muscle decellularization protocols and recellularization with adipose-derived stem cells for tissue engineering.Biologicals : journal of the International Association of Biological Standardization · 2024Article
- Determination of DNA content as quality control in decellularized tissues: challenges and pitfalls.Regenerative biomaterials · 2024Article
- Post-Implantation Inflammatory Responses to Xenogeneic Tissue-Engineered Cartilage Implanted in Rabbit Trachea: The Role of Cultured Chondrocytes in the Modification of Inflammation.International journal of molecular sciences · 2023Article
- The dorsal portion of the bovine diaphragm as a useful tissue for producing a 3D muscle scaffold.Journal of anatomy · 2023Article
- The roles of P-selectin in cancer cachexia.Medical oncology (Northwood, London, England) · 2023Review
- The Use of Collagen Methacrylate in Actuating Polyethylene Glycol Diacrylate-Acrylic Acid Scaffolds for Muscle Regeneration.Annals of biomedical engineering · 2023Article
- Placenta Powder-Infused Thiol-Ene PEG Hydrogels as Potential Tissue Engineering Scaffolds.Biomacromolecules · 2023Article
- Article
- Bio-Engineered Scaffolds Derived from Decellularized Human Esophagus for Functional Organ Reconstruction.Cells · 2022Article
- Biomaterials for Regenerative Medicine in Italy: Brief State of the Art of the Principal Research Centers.International journal of molecular sciences · 2022Review
- Myocyte Culture with Decellularized Skeletal Muscle Sheet with Observable Interaction with the Extracellular Matrix.Bioengineering (Basel, Switzerland) · 2022Article
- Preclinical Development of Bioengineered Allografts Derived from Decellularized Human Diaphragm.Biomedicines · 2022Article
- Perfusion decellularization for vascularized composite allotransplantation.SAGE open medicine · 2022Review
- Characterization of an Innovative Biomaterial Derived From Human Wharton's Jelly as a New Promising Coating for Tissue Engineering Applications.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineered skeletal muscle tissues have been proposed as potential solutions for volumetric muscle losses, and biologic scaffolds have been obtained by decellularization of animal skeletal muscles. The aim of the present work was to analyse the characteristics of a biologic scaffold obtained by decellularization of human skeletal muscles (also through comparison with rats and rabbits) and to evaluate its integration capability in a rabbit model with an abdominal wall defect. Rat, rabbit and human muscle samples were alternatively decellularized with two protocols: n.1, involving sodium deoxycholate and DNase I; n.2, trypsin-EDTA and Triton X-NH4OH. Protocol 2 proved more effective, removing all cellular material and maintaining the three-dimensional networks of collagen and elastic fibers. Ultrastructural analyses with transmission and scanning electron microscopy confirmed the preservation of collagen, elastic fibres, glycosaminoglycans and proteoglycans. Implantation of human scaffolds in rabbits gave good results in terms of integration, although recellularization by muscle cells was not completely achieved. In conclusion, human skeletal muscles may be effectively decellularized to obtain scaffolds preserving the architecture of the extracellular matrix and showing mechanical properties suitable for implantation/integration. Further analyses will be necessary to verify the suitability of these scaffolds for in vitro recolonization by autologous cells before in vivo implantation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.