ArticleSensors (Basel, Switzerland)2015
A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue Virus Serotype 3 Sequences.
Article in Sensors (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 72 citations in OpenAlex.
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- Quantitative detection of dengue serotypes using a smartphone-connected handheld lab-on-chip platform.Frontiers in bioengineering and biotechnology · 2022Article
- COVID-19 challenges: From SARS-CoV-2 infection to effective point-of-care diagnosis by electrochemical biosensing platforms.Biochemical engineering journal · 2021Review
- Toward Development of a Label-Free Detection Technique for Microfluidic Fluorometric Peptide-Based Biosensor Systems.Micromachines · 2021Article
- Portable Sensing Devices for Detection of COVID-19: A Review.IEEE sensors journal · 2021Article
- Recent advances and challenges in electrochemical biosensors for emerging and re-emerging infectious diseases.Journal of electroanalytical chemistry (Lausanne, Switzerland) · 2020Review
- Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art.Biosensors & bioelectronics · 2020Review
- A Label-Free Impedimetric Genosensor for the Nucleic Acid Amplification-Free Detection of Extracted RNA of Dengue Virus.Sensors (Basel, Switzerland) · 2020Article
- Dengue virus: a review on advances in detection and trends - from conventional methods to novel biosensors.Mikrochimica acta · 2019Review
- Optical DNA Biosensor Based on Square-Planar Ethyl Piperidine Substituted Nickel(II) Salphen Complex for Dengue Virus Detection.Sensors (Basel, Switzerland) · 2018Article
- Molecular Biosensors for Electrochemical Detection of Infectious Pathogens in Liquid Biopsies: Current Trends and Challenges.Sensors (Basel, Switzerland) · 2017Review
- Electrochemical Genosensing of Circulating Biomarkers.Sensors (Basel, Switzerland) · 2017Review
- A sensitive DNA capacitive biosensor using interdigitated electrodes.Biosensors & bioelectronics · 2017Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year. Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Due to the lack of fast and inexpensive methods available for the identification of dengue serotypes, herein we report the development of an electrochemical DNA biosensor for the detection of sequences of dengue virus serotype 3 (DENV-3). DENV-3 probe was designed using bioinformatics software and differential pulse voltammetry (DPV) was used for electrochemical analysis. The results showed that a 22-m sequence was the best DNA probe for the identification of DENV-3. The optimum concentration of the DNA probe immobilized onto the electrode surface is 500 nM and a low detection limit of the system (3.09 nM). Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions. Therefore, the application of DNA biosensors for diagnostics at the molecular level may contribute to future advances in the implementation of specific, effective and rapid detection methods for the diagnosis dengue viruses.
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