Evidence map›Paper›PMID 26140186›Full record

ArticleAvicenna journal of medical biotechnology

Maternal Supplementary Folate Intake, Methylenetetrahydrofolate Reductase (MTHFR) C677T and A1298C Polymorphisms and the Risk of Orofacial Cleft in Iranian Children.

Asghar Ebadifar, Hamid Reza KhorramKhorshid, Koorosh Kamali, Mehdi Salehi Zeinabadi, Tayyebeh Khoshbakht, Nazila Ameli

Registry-linked trialOpen access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Avicenna journal of medical biotechnology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03725878 (Effectiveness of Comprehensive Tertiary Interventions on Incidence and Clinical Outcomes of Birth Defects), which is not on this map. Cited by 7 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 2 pooled it
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03725878 nacompletednot on this map

Effectiveness of Comprehensive Tertiary Interventions on Incidence and Clinical Outcomes of Birth Defects : a Cluster Randomisation Intervention Trial

TypeinterventionalSponsorChildren's Hospital of Fudan UniversityRan2018 to 2024Enrolled4,808ConditionsBirth DefectsArmsStandard tertiary interventions of birth defects, Additional preconception health care, Additional health care procedures during and after pregnancy
3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 2 syntheses or guidelines pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Asghar EbadifarDentofacial Deformities Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hamid Reza KhorramKhorshidGenetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
Koorosh KamaliReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Mehdi Salehi ZeinabadiPediatric Department, Dental School, Semnan University of Medical Sciences, Semnan, Iran.
Tayyebeh KhoshbakhtGenetic Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
Nazila AmeliOrthodontic Department, Dental school, Semnan University of Medical Sciences, Semnan, Iran.
University of Social Welfare and Rehabilitation Sciences · IRBiotechnology Research Center · IRShahid Beheshti University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe purpose of this study was to describe the association of MTHFR gene single nucleotide polymorphisms (C677T and A1298C) and maternal supplementary folate intake with orofacial clefts in the Iranian population.

methodsIn this case-control study, peripheral venous blood was taken from 65 patients with orofacial clefts and 215 unaffected controls for DNA extraction and kept in EDTA for further analysis. The genotyping was carried out using Polymerase Chain Reaction (PCR) followed by Restriction Fragment Length Polymorphism (RFLP) and gel electrophoresis. Data were analyzed using Chi square test and logistic regression tests.

resultsGenotype frequencies of 677TT were reported to be 13.5 and 36.1% in controls and CL/P patients, respectively, which showed a significant difference compared to CC as reference (OR=4.118; 95% CI=1.997-8.492; p=0.001). Conversely, 1298CC with frequencies of 10.8 and 12.7% in controls and patients, respectively, showed no significant difference compared to AA (OR=2.359; 95% CI=0.792-7.023; p=0.123). Comparing patients whose mothers did not report the folate supplement intake during pregnancy, to controls, it was observed that lack of folate intake was a predisposing factor for having a child with oral clefts (OR=5/718, p=0.000).

conclusionChildren carrying the 677TT variant of the MTHFR gene may have an increased risk of CL/P. In addition, the finding that the risk associated with this allele was obviously higher when the mothers didn't use folic acid, supports the hypothesis that folic acid may play a role in the etiology of CL/P.

Indexed as

Cleft lipCleft palateGenesPolymorphism

Identifiers

PMID26140186
PMCPMC4483319
OpenAlexW2134452675

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.