ArticleThe Journal of biological chemistry2015
Insights into Flavin-based Electron Bifurcation via the NADH-dependent Reduced Ferredoxin:NADP Oxidoreductase Structure.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 106 citations in OpenAlex.
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- The vast landscape of carbohydrate fermentation in prokaryotes.FEMS microbiology reviews · 2024Review
- Structures and Electron Transport Paths in the Four Families of Flavin-Based Electron Bifurcation Enzymes.Sub-cellular biochemistry · 2024Review
- Characterization of the Membrane-Associated Electron-Bifurcating Flavoenzyme EtfABCX from the Hyperthermophilic BacteriumBiochemistry · 2023Article
- Rapid-reaction kinetics of the bifurcating NADThe Journal of biological chemistry · 2023Article
- Molecular architecture and electron transfer pathway of the Stn family transhydrogenase.Nature communications · 2023Article
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- Molecular Basis of the Electron Bifurcation Mechanism in the [FeFe]-Hydrogenase Complex HydABC.Journal of the American Chemical Society · 2023Article
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- Site-Differentiated Iron-Sulfur Cluster Ligation Affects Flavin-Based Electron Bifurcation Activity.Metabolites · 2022Article
- A Cytoplasmic NAD(P)H-Dependent Polysulfide Reductase with Thiosulfate Reductase Activity from the Hyperthermophilic Bacterium Thermotoga maritima.Microbiology spectrum · 2022Article
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- A site-differentiated [4Fe-4S] cluster controls electron transfer reactivity ofChemical science · 2022Article
- Crystallographic snapshots of a BNature · 2022Article
- An Abundant and Diverse New Family of Electron Bifurcating Enzymes With a Non-canonical Catalytic Mechanism.Frontiers in microbiology · 2022Article
- Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea.PLoS biology · 2022Review
- Customized exogenous ferredoxin functions as an efficient electron carrier.Bioresources and bioprocessing · 2021Article
- Iron-sulfur flavoenzymes: the added value of making the most ancient redox cofactors and the versatile flavins work together.Open biology · 2021Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NADH-dependent reduced ferredoxin:NADP oxidoreductase (NfnAB) is found in the cytoplasm of various anaerobic bacteria and archaea. The enzyme reversibly catalyzes the endergonic reduction of ferredoxin with NADPH driven by the exergonic transhydrogenation from NADPH onto NAD(+). Coupling is most probably accomplished via the mechanism of flavin-based electron bifurcation. To understand this process on a structural basis, we heterologously produced the NfnAB complex of Thermotoga maritima in Escherichia coli, provided kinetic evidence for its bifurcating behavior, and determined its x-ray structure in the absence and presence of NADH. The structure of NfnAB reveals an electron transfer route including the FAD (a-FAD), the [2Fe-2S] cluster of NfnA and the FAD (b-FAD), and the two [4Fe-4S] clusters of NfnB. Ferredoxin is presumably docked onto NfnB close to the [4Fe-4S] cluster distal to b-FAD. NAD(H) binds to a-FAD and NADP(H) consequently to b-FAD, which is positioned in the center of the NfnAB complex and the site of electron bifurcation. Arg(187) is hydrogen-bonded to N5 and O4 of the bifurcating b-FAD and might play a key role in adjusting a low redox potential of the FADH(•)/FAD pair required for ferredoxin reduction. A mechanism of FAD-coupled electron bifurcation by NfnAB is proposed.
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