ArticleJournal of microscopy2015
Combining quantitative 2D and 3D image analysis in the serial block face SEM: application to secretory organelles of pancreatic islet cells.
Article in Journal of microscopy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Islet ultrastructure: past achievements and future directions.Diabetologia · 2026Review
- Biological volume EM with focused Ga ion beam depends on formation of radiation-resistant Ga-rich layer at block face.Research square · 2025Article
- Article
- 3D FIB-SEM reconstruction of microtubule-organelle interaction in whole primary mouse β cells.The Journal of cell biology · 2021Article
- Determination of secretory granule maturation times in pancreatic islet β-cells by serial block-face electron microscopy.Journal of structural biology · 2020Article
- Quantitative method for estimating stain density in electron microscopy of conventionally prepared biological specimens.Journal of microscopy · 2020Article
- Biological serial block face scanning electron microscopy at improved z-resolution based on Monte Carlo model.Scientific reports · 2018Article
- Comparison of 3D cellular imaging techniques based on scanned electron probes: Serial block face SEM vs. Axial bright-field STEM tomography.Journal of structural biology · 2018Article
- Quantifying Golgi structure using EM: combining volume-SEM and stereology for higher throughput.Histochemistry and cell biology · 2017Review
- Golgi proteins in circulating human platelets are distributed across non-stacked, scattered structures.Platelets · 2017Article
- The cellular basis of platelet secretion: Emerging structure/function relationships.Platelets · 2017Review
- β-arrestin-2 is an essential regulator of pancreatic β-cell function under physiological and pathophysiological conditions.Nature communications · 2017Article
- Using electron microscopes to look into the lung.Histochemistry and cell biology · 2016Review
- Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
A combination of two-dimensional (2D) and three-dimensional (3D) analyses of tissue volume ultrastructure acquired by serial block face scanning electron microscopy can greatly shorten the time required to obtain quantitative information from big data sets that contain many billions of voxels. Thus, to analyse the number of organelles of a specific type, or the total volume enclosed by a population of organelles within a cell, it is possible to estimate the number density or volume fraction of that organelle using a stereological approach to analyse randomly selected 2D block face views through the cells, and to combine such estimates with precise measurement of 3D cell volumes by delineating the plasma membrane in successive block face images. The validity of such an approach can be easily tested since the entire 3D tissue volume is available in the serial block face scanning electron microscopy data set. We have applied this hybrid 3D/2D technique to determine the number of secretory granules in the endocrine α and β cells of mouse pancreatic islets of Langerhans, and have been able to estimate the total insulin content of a β cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.