ArticleHistochemistry and cell biology2015
Artifacts in single-molecule localization microscopy.
Article in Histochemistry and cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Noninvasive methods to monitor dynamic single-cell events.Trends in cell biology · 2026Review
- Phalloidin-PAINT: Enhanced quantitative nanoscale imaging of F-actin.Biophysical journal · 2024Article
- Membrane topography and the overestimation of protein clustering in single molecule localisation microscopy - identification and correction.Communications biology · 2024Article
- Quantitative Superresolution Imaging of F-Actin in the Cell Body and Cytoskeletal Protrusions Using Phalloidin-Based Single-Molecule Labeling and Localization Microscopy.bioRxiv : the preprint server for biology · 2024Article
- Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy.Neurophotonics · 2023Article
- Application of Super-resolution SPEED Microscopy in the Study of Cellular Dynamics.Chemical & biomedical imaging · 2023Review
- Fluorescence radial fluctuation enables two-photon super-resolution microscopy.Frontiers in cellular neuroscience · 2023Article
- Fluorescent Nanoparticles for Super-Resolution Imaging.Chemical reviews · 2022Review
- Approach to map nanotopography of cell surface receptors.Communications biology · 2022Article
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- Sub-diffraction error mapping for localisation microscopy images.Nature communications · 2021Article
- DETECTOR: structural information guided artifact detection for super-resolution fluorescence microscopy image.Biomedical optics express · 2021Article
- Detecting structural heterogeneity in single-molecule localization microscopy data.Nature communications · 2021Article
- Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries.Biotechnology for biofuels · 2021Review
- Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes.European journal of cell biology · 2020Article
- Spectral cross-cumulants for multicolor super-resolved SOFI imaging.Nature communications · 2020Article
- Super-beacons: Open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy.Traffic (Copenhagen, Denmark) · 2020Article
- Using Expansion Microscopy to Visualize and Characterize the Morphology of Mitochondrial Cristae.Frontiers in cell and developmental biology · 2020Article
- The Decade of Super-Resolution Microscopy of the Presynapse.Frontiers in synaptic neuroscience · 2020Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-molecule localization microscopy provides subdiffraction resolution images with virtually molecular resolution. Through the availability of commercial instruments and open-source reconstruction software, achieving super resolution is now public domain. However, despite its conceptual simplicity, localization microscopy remains prone to user errors. Using direct stochastic optical reconstruction microscopy, we investigate the impact of irradiation intensity, label density and photoswitching behavior on the distribution of membrane proteins in reconstructed super-resolution images. We demonstrate that high emitter densities in combination with inappropriate photoswitching rates give rise to the appearance of artificial membrane clusters. Especially, two-dimensional imaging of intrinsically three-dimensional membrane structures like microvilli, filopodia, overlapping membranes and vesicles with high local emitter densities is prone to generate artifacts. To judge the quality and reliability of super-resolution images, the single-molecule movies recorded to reconstruct the images have to be carefully investigated especially when investigating membrane organization and cluster analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.