Evidence map›Paper›PMID 26137941›Full record

ArticleBMC gastroenterology2015

Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn's disease.

Timothy R Elliott, Neil B Rayment, Barry N Hudspith, Rebecca E Hands, Kirstin Taylor, Gareth C Parkes, Natalie J Prescott, Liljana Petrovska, John Hermon-Taylor, Jonathan Brostoff and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC gastroenterology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Timothy R ElliottDiabetes and Nutritional Sciences Division, King's College London, Franklin Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK. elliott_timothy@hotmail.com.
Neil B RaymentDiabetes and Nutritional Sciences Division, King's College London, Franklin Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK. neil.rayment@kcl.ac.uk.
Barry N HudspithDiabetes and Nutritional Sciences Division, King's College London, Franklin Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK. barry.hudspith@kcl.ac.uk.
Rebecca E HandsBlizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Whitechapel, London, E1 1BB, UK. r.e.hands@qmul.ac.uk.
Kirstin TaylorDepartment of Gastroenterology, Guy's and St Thomas' NHS Foundation Trust, St Thomas' Hospital, London, SE1 7EH, UK. kirstin.taylor@kcl.ac.uk.
Gareth C ParkesDiabetes and Nutritional Sciences Division, King's College London, Franklin Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK. gareth.parkes@kcl.ac.uk.
Natalie J PrescottDepartment of Medical and Molecular Genetics, King's College London School of Medicine, Guy's Hospital, London, SE1 9RT, UK. natalie.prescott@kcl.ac.uk.
Liljana PetrovskaDiabetes and Nutritional Sciences Division, King's College London, Franklin Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK. Liljana.petrovska@ahvla.gsi.gov.uk.
John Hermon-TaylorDiabetes and Nutritional Sciences Division, King's College London, Franklin Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK. j.hermon@kcl.ac.uk.
Jonathan BrostoffDiabetes and Nutritional Sciences Division, King's College London, Franklin Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK. j.brostoff@virgin.net.
Alex BoussioutasDepartment of Medicine, University of Melbourne, Melbourne, Australia. alexb@unimelb.edu.au.
Christopher G MathewDepartment of Medical and Molecular Genetics, King's College London School of Medicine, Guy's Hospital, London, SE1 9RT, UK. christopher.mathew@kcl.ac.uk.
Stephen A BustinPostgraduate Medical Institute, Faculty of Medical Science, Anglia Ruskin University, Chelmsford, Essex, UK. stephen.bustin@anglia.ac.uk.
Jeremy D SandersonDiabetes and Nutritional Sciences Division, King's College London, Franklin Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK. jeremy.sanderson@kcl.ac.uk.
King's College London · GBGuy's Hospital · GBThe University of Melbourne · AUAnglia Ruskin University · GBQueen Mary University of London · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAbnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn's disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage.

methodsMucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR.

resultsE. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls.

conclusionIn CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation.

Indexed as

PhenotypeAdultAgedBiomarkersCase-Control StudiesColitis, UlcerativeCrohn DiseaseCytokinesEscherichia coliFemaleHumansIntestinal MucosaMacrophagesMaleMiddle AgedBiomarkersCytokines

Identifiers

PMID26137941
PMCPMC4490755
OpenAlexW1553556747

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.