Evidence map›Paper›PMID 26137499›Full record

ArticleBio-protocol2015

Generating Isogenic Deletions (Knockouts) in

Xiaojun Wu, Guoping Ren, Jason F Huntley

Open access · greenAbstract read
In one paragraph

Article in Bio-protocol, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. APathogens (Basel, Switzerland) · 2020
    Article
  2. The orange spotted cockroach (Proceedings of the West Virginia Academy of Science · 2017
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Xiaojun Wu *Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, Toledo, USA.
Guoping Ren *Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, Toledo, USA.
Jason F HuntleyDepartment of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, Toledo, USA.
University of Toledo · US

Funding

Preclinical Development of a Tularemia VaccineR01AI093351 · NIAID · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI HUNTLEY, JASON F · 2011 to 2015
$1.9M
Francisella tularensis intracellularly-induced outer membrane proteinsK22AI083372 · NIAID · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI HUNTLEY, JASON F · 2010 to 2011
$266k
NIAID NIH HHS K22 AI083372NIAID NIH HHS R01 AI093351
6 · The paper itself

Abstract

Generating bacterial gene deletion mutants, also known as knockouts (KOs), is a powerful tool to investigate individual gene functions. However, fastidious bacteria such as

Identifiers

PMID26137499
PMCPMC4484883
OpenAlexW2408791020

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.