ArticleBiomedical optics express2015
Deep, high contrast microscopic cell imaging using three-photon luminescence of β-(NaYF4:Er(3+)/NaYF4) nanoprobe excited by 1480-nm CW laser of only 1.5-mW.
Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Excitation Pulse Duration Response of Upconversion Nanoparticles and Its Applications.The journal of physical chemistry letters · 2022Article
- Fluorescent Nanoparticles for Super-Resolution Imaging.Chemical reviews · 2022Review
- Light-sheet microscopy in the near-infrared II window.Nature methods · 2019Article
- Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles.Nature communications · 2017Article
- Designed Er(3+)-singly doped NaYF4 with double excitation bands for simultaneous deep macroscopic and microscopic upconverting bioimaging.Biomedical optics express · 2016Article
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Authors and funding
6 authors.
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Abstract
It is challenging to achieve deep microscopic imaging for the strong scattering in biotissue. An efficient three-photon luminescence can effectively increase the penetration depth. Here we report that β-NaYF4: Er(3+)/NaYF4 UCNPs were excited by a 1480-nm CW-laser and emitted 543/653-nm light through a three-photon process. With the merit of the hexagonal crystal phase, sub-milliwatt laser power was utilized to excite the UCNP-probed cells to minimize the heating effect. The polymer-coated UCNPs were shown to be harmless to cells. The deep, high contrast in vitro microscopic imaging was implemented through an artificial phantom. Imaging depth of 800 μm was achieved using only 1.5 mW excitation and a 0.7 NA objective. The green/red emission intensities ratio after penetrating the phantom was studied, indicating that longer emission wavelength is preferred for deep multiphoton microscopy. The proposed and demonstrated β-UCNPs would have great potential in three-photon microscopy.
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