ArticleBiotechnology for biofuels2015
Substrate specificity and regioselectivity of fungal AA9 lytic polysaccharide monooxygenases secreted by Podospora anserina.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
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Who cites it
107 citing papers in PubMed, 241 citations in OpenAlex.
- Electrochemical Insight into the Copper Redox Chemistry and HACS electrochemistry · 2026Article
- Anions and citrate inhibit LsAA9A, a lytic polysaccharide monooxygenase (LPMO).The FEBS journal · 2025Article
- Functional variation among LPMOs revealed by the inhibitory effects of cyanide and buffer ions.FEBS letters · 2025Article
- LPMO-Catalyzed Oxidation of Cellulosic Fibers with Controlled Addition of a Reductant and HACS sustainable chemistry & engineering · 2025Article
- Design of Plastic Binding Lytic Polysaccharide Monooxygenases via Modular Engineering.Chem & bio engineering · 2024Article
- Phylogenomics reveals the evolutionary origins of lichenization in chlorophyte algae.Nature communications · 2024Article
- The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Expanding the catalytic landscape of metalloenzymes with lytic polysaccharide monooxygenases.Nature reviews. Chemistry · 2024Review
- Insights into peculiar fungal LPMO family members holding a short C-terminal sequence reminiscent of phosphate binding motifs.Scientific reports · 2023Article
- Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce.Biotechnology for biofuels and bioproducts · 2023Article
- Revisiting the role of electron donors in lytic polysaccharide monooxygenase biochemistry.Essays in biochemistry · 2023Review
- Recent advances in the efficient degradation of lignocellulosic metabolic networks by lytic polysaccharide monooxygenase.Acta biochimica et biophysica Sinica · 2023Review
- Recent Theoretical Insights into the Oxidative Degradation of Biopolymers and Plastics by Metalloenzymes.International journal of molecular sciences · 2023Review
- Molecular Mechanism of Substrate Oxidation in Lytic Polysaccharide Monooxygenases: Insight from Theoretical Investigations.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Review
- Closing the Nutrient Loop-The New Approaches to Recovering Biomass Minerals during the Biorefinery Processes.International journal of environmental research and public health · 2023Review
- Enzymatic debranching is a key determinant of the xylan-degrading activity of family AA9 lytic polysaccharide monooxygenases.Biotechnology for biofuels and bioproducts · 2023Article
- Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Deletion of AA9 Lytic Polysaccharide Monooxygenases Impacts A. nidulans Secretome and Growth on Lignocellulose.Microbiology spectrum · 2022Article
- AA15 lytic polysaccharide monooxygenase is required for efficient chitinous cuticle turnover during insect molting.Communications biology · 2022Article
- Comparison of Six Lytic Polysaccharide Monooxygenases fromApplied and environmental microbiology · 2022Article
47 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe understanding of enzymatic polysaccharide degradation has progressed intensely in the past few years with the identification of a new class of fungal-secreted enzymes, the lytic polysaccharide monooxygenases (LPMOs) that enhance cellulose conversion. In the fungal kingdom, saprotrophic fungi display a high number of genes encoding LPMOs from family AA9 but the functional relevance of this redundancy is not fully understood.
resultsIn this study, we investigated a set of AA9 LPMOs identified in the secretomes of the coprophilous ascomycete Podospora anserina, a biomass degrader of recalcitrant substrates. Their activity was assayed on cellulose in synergy with the cellobiose dehydrogenase from the same organism. We showed that the total release of oxidized oligosaccharides from cellulose was higher for PaLPMO9A, PaLPMO9E, and PaLPMO9H that harbored a carbohydrate-binding module from the family CBM1. Investigation of their regioselective mode of action revealed that PaLPMO9A and PaLPMO9H oxidatively cleaved at both C1 and C4 positions while PaLPMO9E released only C1-oxidized products. Rapid cleavage of cellulose was observed using PaLPMO9H that was the most versatile in terms of substrate specificity as it also displayed activity on cello-oligosaccharides and β-(1,4)-linked hemicellulose polysaccharides (e.g., xyloglucan, glucomannan).
conclusionsThis study provides insights into the mode of cleavage and substrate specificities of fungal AA9 LPMOs that will facilitate their application for the development of future biorefineries.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.