ArticleAngewandte Chemie (International ed. in English)2015
Chemical Evolution of a Bacterial Proteome.
Article in Angewandte Chemie (International ed. in English), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 80 citations in OpenAlex.
- Advances in adaptive laboratory evolution applications forSynthetic and systems biotechnology · 2025Review
- Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:Chemical reviews · 2025Review
- Engineered mRNA-ribosome fusions for facile biosynthesis of selenoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Non-Canonical Amino Acids in Analyses of Protease Structure and Function.International journal of molecular sciences · 2023Review
- Catecholamine Derivatives as Novel Crosslinkers for the Synthesis of Versatile Biopolymers.Journal of functional biomaterials · 2023Review
- Recent advances in screening amino acid overproducers.Engineering microbiology · 2023Review
- Effect of Non-Lethal Selection on Spontaneous Revertants of Frameshift Mutations: The Escherichia coli hisF CaseMicroorganisms · 2022Article
- Non-canonical Amino Acid Substrates of E. coli Aminoacyl-tRNA Synthetases.Chembiochem : a European journal of chemical biology · 2022Review
- How To Quantify a Genetic Firewall? A Polarity-Based Metric for Genetic Code Engineering.Chembiochem : a European journal of chemical biology · 2021Article
- Multiomics Analysis Provides Insight into the Laboratory Evolution ofACS central science · 2021Article
- Using Genetic Code Expansion for Protein Biochemical Studies.Frontiers in bioengineering and biotechnology · 2020Review
- In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation.International journal of molecular sciences · 2019Article
- Plasticity and Constraints of tRNA Aminoacylation Define Directed Evolution of Aminoacyl-tRNA Synthetases.International journal of molecular sciences · 2019Review
- Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications.Applied microbiology and biotechnology · 2019Review
- Review
- Utilization of rare codon-rich markers for screening amino acid overproducers.Nature communications · 2018Article
- Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence.Journal of visualized experiments : JoVE · 2018Article
- Evolving Bacterial Fitness with an Expanded Genetic Code.Scientific reports · 2018Article
- Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications.Journal of biological engineering · 2018Review
- Rewiring protein synthesis: From natural to synthetic amino acids.Biochimica et biophysica acta. General subjects · 2017Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20,899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.