ArticlePloS one2015
CRISPR Content Correlates with the Pathogenic Potential of Escherichia coli.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- The effects of bacteriophage cocktail treatment on healthy gut microbiota: anMicrobial genomics · 2026Article
- LysR-type regulator LrhA promotes CRISPR-Cas immunity in Escherichia coli.Nucleic acids research · 2026Article
- Diversity of CRISPR-Cas Systems Identified in UrologicalMicroorganisms · 2025Article
- From the Gut to the Brain: Transcriptomic Insights into Neonatal MeningitisPathogens (Basel, Switzerland) · 2025Article
- Antibiotic-ResistantMicroorganisms · 2025Review
- Two multidrug-resistantHeliyon · 2024Article
- Interrelation Between Pathoadaptability Factors and Crispr-Element Patterns in the Genomes ofPathogens (Basel, Switzerland) · 2024Article
- Escherichia coli CRISPR arrays from early life fecal samples preferentially target prophages.The ISME journal · 2024Article
- Comparative study of virulence potential, phylogenetic origin, CRISPR-Frontiers in microbiology · 2023Article
- Correlation between CRISPR Loci Diversity in Three Enterobacterial Taxa.International journal of molecular sciences · 2022Article
- Distribution of CRISPR inAnimals : an open access journal from MDPI · 2022Article
- Review of CRISPR-Cas Systems inInternational journal of microbiology · 2022Review
- Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections.Journal of nanobiotechnology · 2021Review
- Digging into the lesser-known aspects of CRISPR biology.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- The phylogenomics of CRISPR-Cas system and revelation of its features in Salmonella.Scientific reports · 2020Article
- Identification of Spacer and Protospacer Sequence Requirements in the Vibrio cholerae Type I-E CRISPR/Cas System.mSphere · 2020Article
- One-Day Construction of Multiplex Arrays to Harness Natural CRISPR-Cas Systems.ACS synthetic biology · 2020Article
- Survey and Sequence Characterization of Bovine Mastitis-AssociatedFrontiers in veterinary science · 2020Article
- Article
- The CRISPR conundrum: evolve and maybe die, or survive and risk stagnation.Microbial cell (Graz, Austria) · 2018Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Guide RNA molecules (crRNA) produced from clustered regularly interspaced short palindromic repeat (CRISPR) arrays, altogether with effector proteins (Cas) encoded by cognate cas (CRISPR associated) genes, mount an interference mechanism (CRISPR-Cas) that limits acquisition of foreign DNA in Bacteria and Archaea. The specificity of this action is provided by the repeat intervening spacer carried in the crRNA, which upon hybridization with complementary sequences enables their degradation by a Cas endonuclease. Moreover, CRISPR arrays are dynamic landscapes that may gain new spacers from infecting elements or lose them for example during genome replication. Thus, the spacer content of a strain determines the diversity of sequences that can be targeted by the corresponding CRISPR-Cas system reflecting its functionality. Most Escherichia coli strains possess either type I-E or I-F CRISPR-Cas systems. To evaluate their impact on the pathogenicity of the species, we inferred the pathotype and pathogenic potential of 126 strains of this and other closely related species and analyzed their repeat content. Our results revealed a negative correlation between the number of I-E CRISPR units in this system and the presence of pathogenicity traits: the median number of repeats was 2.5-fold higher for commensal isolates (with 29.5 units, range 0-53) than for pathogenic ones (12.0, range 0-42). Moreover, the higher the number of virulence factors within a strain, the lower the repeat content. Additionally, pathogenic strains of distinct ecological niches (i.e., intestinal or extraintestinal) differ in repeat counts. Altogether, these findings support an evolutionary connection between CRISPR and pathogenicity in E. coli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.