ArticleMolecular plant pathology2016
Polycistronic artificial miRNA-mediated resistance to Wheat dwarf virus in barley is highly efficient at low temperature.
Article in Molecular plant pathology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 102 citations in OpenAlex.
- What Do We Know about Barley miRNAs?International journal of molecular sciences · 2022Pooled it
- MicroRNA-Induced Gene Silencing (MIGS): A Tool for Multi-Gene Silencing and Targeting Viruses in Plants.Plant biotechnology journal · 2026Article
- Integrative Roles of miRNAs and circRNAs in Plant Antiviral Gene Regulation and Autophagy.Plants (Basel, Switzerland) · 2025Review
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Article
- Remote precursor elements can modulate RNA induced silencing complex-loading efficiency of miR168 in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- The Emerging Applications of Artificial MicroRNA-Mediated Gene Silencing in Plant Biotechnology.Non-coding RNA · 2025Review
- Promising Biotechnological Applications of the Artificial Derivatives Designed and Constructed from Plant microRNA Genes.Plants (Basel, Switzerland) · 2025Review
- Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant.Transgenic research · 2024Article
- SilencingPotato research · 2023Article
- Targeting of genomic and negative-sense strands of viral RNA contributes to antiviral resistance mediated by artificial miRNAs and promotes the emergence of complex viral populations.Molecular plant pathology · 2022Article
- Controlled RISC loading efficiency of miR168 defined by miRNA duplex structure adjusts ARGONAUTE1 homeostasis.Nucleic acids research · 2021Article
- MicroRNA-mediated bioengineering for climate-resilience in crops.Bioengineered · 2021Review
- Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber.International journal of molecular sciences · 2021Article
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Optimized RNA-Silencing Strategies forPlants (Basel, Switzerland) · 2021Article
- Roles of small RNAs in crop disease resistance.Stress biology · 2021Review
- Transcriptomic analysis to reveal the differentially expressed miRNA targets and their miRNAs in response to Ralstonia solanacearum in ginger species.BMC plant biology · 2021Article
- Plant responses to geminivirus infection: guardians of the plant immunity.Virology journal · 2021Review
- Biotechnological Resources to Increase Disease-Resistance by Improving Plant Immunity: A Sustainable Approach to Save Cereal Crop Production.Plants (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infection of Wheat dwarf virus (WDV) strains on barley results in dwarf disease, imposing severe economic losses on crop production. As the natural resistance resources against this virus are limited, it is imperative to elaborate a biotechnological approach that will provide effective and safe immunity to a wide range of WDV strains. Because vector insect-mediated WDV infection occurs during cool periods in nature, it is important to identify a technology which is effective at lower temperature. In this study, we designed artificial microRNAs (amiRNAs) using a barley miRNA precursor backbone, which target different conservative sequence elements of the WDV strains. Potential amiRNA sequences were selected to minimize the off-target effects and were tested in a transient sensor system in order to select the most effective constructs at low temperature. On the basis of the data obtained, a polycistronic amiRNA precursor construct (VirusBuster171) was built expressing three amiRNAs simultaneously. The construct was transformed into barley under the control of a constitutive promoter. The transgenic lines were kept at 12-15 °C to mimic autumn and spring conditions in which major WDV infection and accumulation take place. We were able to establish a stable barley transgenic line displaying resistance to insect-mediated WDV infection. Our study demonstrates that amiRNA technology can be an efficient tool for the introduction of highly efficient resistance in barley against a DNA virus belonging to the Geminiviridae family, and this resistance is effective at low temperature where the natural insect vector mediates the infection process.
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