ArticleJournal of clinical microbiology2015
Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Whole-Genome Sequence-Based Typing of Listeria monocytogenes.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 180 papers.
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180 citing papers in PubMed.
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- Emergence and genomic characterization of Listeria monocytogenes causing human listeriosis in Pakistan, 2017-2023.BMC microbiology · 2026Article
- Whole Genome Sequence-Based Surveillance of Human ClinicalOpen forum infectious diseases · 2026Article
- Assessment of the Microbiological Quality of Raw Milk Sold Through Vending Machines at the Farm Level in Switzerland.Pathogens (Basel, Switzerland) · 2026Article
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- BV-BRC: a unified bacterial and viral bioinformatics resource with expanded functionality and AI integration.Nucleic acids research · 2026Article
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- Genomic characterization ofApplied and environmental microbiology · 2025Article
- The European Union One Health 2024 Zoonoses Report.EFSA journal. European Food Safety Authority · 2025Article
- Listeriosis.Nature reviews. Disease primers · 2025Review
- Multi-country and intersectoral assessment of cluster congruence between pipelines for genomics surveillance of foodborne pathogens.Nature communications · 2025Article
- Outbreak of listeriosis associated with consumption of deli meats in a hospital, Germany, February to March 2023.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- A core genome MLST scheme for Borrelia burgdorferi sensu lato improves insights into the evolutionary history of the species complex.Cell reports methods · 2025Article
- From Traditional Typing to Genomic Precision: Whole-Genome Sequencing ofFoods (Basel, Switzerland) · 2025Article
- Assessing mutation accumulation in DNA repair-deficientFrontiers in cellular and infection microbiology · 2025Article
120 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Whole-genome sequencing (WGS) has emerged today as an ultimate typing tool to characterize Listeria monocytogenes outbreaks. However, data analysis and interlaboratory comparability of WGS data are still challenging for most public health laboratories. Therefore, we have developed and evaluated a new L. monocytogenes typing scheme based on genome-wide gene-by-gene comparisons (core genome multilocus the sequence typing [cgMLST]) to allow for a unique typing nomenclature. Initially, we determined the breadth of the L. monocytogenes population based on MLST data with a Bayesian approach. Based on the genome sequence data of representative isolates for the whole population, cgMLST target genes were defined and reappraised with 67 L. monocytogenes isolates from two outbreaks and serotype reference strains. The Bayesian population analysis generated five L. monocytogenes groups. Using all available NCBI RefSeq genomes (n = 36) and six additionally sequenced strains, all genetic groups were covered. Pairwise comparisons of these 42 genome sequences resulted in 1,701 cgMLST targets present in all 42 genomes with 100% overlap and ≥90% sequence similarity. Overall, ≥99.1% of the cgMLST targets were present in 67 outbreak and serotype reference strains, underlining the representativeness of the cgMLST scheme. Moreover, cgMLST enabled clustering of outbreak isolates with ≤10 alleles difference and unambiguous separation from unrelated outgroup isolates. In conclusion, the novel cgMLST scheme not only improves outbreak investigations but also enables, due to the availability of the automatically curated cgMLST nomenclature, interlaboratory exchange of data that are crucial, especially for rapid responses during transsectorial outbreaks.
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