Evidence map›Paper›PMID 2613554›Full record

ArticleHistochemistry1989

Protein-gold complexes as neuronal markers for long-term tracing studies.

D Menétrey, J de Pommery

Abstract read
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In one paragraph

Article in Histochemistry, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

D MenétreyINSERM, Physiopharmacologie du système nerveux, Paris, France.
J de Pommery

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we have tested the possible use of protein-gold complexes as neuronal markers for long-term tracing studies in rat. The tracer we have used consisted of colloidal gold particles coupled to wheat-germ agglutinin apohorseradish peroxidase conjugate (WGA-apoHRP). The neuronal labeling was studied for survival periods of up to nineteen months following injection in the central nervous system. Maximal visualization of the gold particles was achieved through gold silver intensification. The tracer could be detected throughout the entire range of periods considered. The injection site consisted of a dense black core and retrogradely labeled cells were characterized by round black granules over the cell body. The retrogradely labeled cells were cytochemically characterized by demonstrating their transmitter content. Thus protein-gold complexes may be used as long-term neuronal markers compatible with the persistance of the vital functions of the labeled cells.

Indexed as

Wheat Germ Agglutinin-Horseradish Peroxidase ConjugateAnimalsBiological TransportFrozen SectionsHorseradish PeroxidaseImmunohistochemistryInjections, SpinalNeuronsPeroxidasesRatsRats, Inbred StrainsRed NucleusSomatosensory CortexTime FactorsWheat Germ AgglutininsHorseradish PeroxidasePeroxidaseswheat germ agglutinin-apohorseradish peroxidase conjugate gold complexWheat Germ Agglutinin-Horseradish Peroxidase ConjugateWheat Germ Agglutinins

Identifiers

PMID2613554

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.