ArticleMolecular medicine reports2015
Presence and function of microRNA-92a in chondrogenic ATDC5 and adipose-derived mesenchymal stem cells.
Article in Molecular medicine reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Enhancing Cartilage Repair: Surgical Approaches, Orthobiologics, and the Promise of Exosomes.Life (Basel, Switzerland) · 2024Review
- State of the Art: The Immunomodulatory Role of MSCs for Osteoarthritis.International journal of molecular sciences · 2022Review
- Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential.International journal of molecular sciences · 2021Review
- Mesenchymal Stem Cell-Derived Exosomes and MicroRNAs in Cartilage Regeneration: Biogenesis, Efficacy, miRNA Enrichment and Delivery.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Diagnostic and Therapeutic Role of Extracellular Vesicles in Articular Cartilage Lesions and Degenerative Joint Diseases.Frontiers in bioengineering and biotechnology · 2021Review
- Engineered-extracellular vesicles as an optimistic tool for microRNA delivery for osteoarthritis treatment.Cellular and molecular life sciences : CMLS · 2021Review
- Identifying the Potential Differentially Expressed miRNAs and mRNAs in Osteonecrosis of the Femoral Head Based on Integrated Analysis.Clinical interventions in aging · 2021Article
- Research Progress on Stem Cell Therapies for Articular Cartilage Regeneration.Stem cells international · 2021Review
- MiR-520d-5p modulates chondrogenesis and chondrocyte metabolism through targeting HDAC1.Aging · 2020Article
- Extracellular vesicles: Potential role in osteoarthritis regenerative medicine.Journal of orthopaedic translation · 2020Review
- Identification of differentially expressed miRNAs associated with thermal injury in epidermal stem cells based on RNA-sequencing.Experimental and therapeutic medicine · 2020Article
- Potential Application of Human β-Defensin 4 in Dental Pulp Repair.Frontiers in physiology · 2020Article
- miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2.International journal of molecular medicine · 2018Article
- Cyclic Tensile Strain Upregulates Pro-Inflammatory Cytokine Expression Via FAK-MAPK Signaling in Chondrocytes.Inflammation · 2018Article
- [Research progress of miRNA regulation in differentiation of adipose-derived stem cells].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to investigate the presence and biological function of microRNA-92a (miR-92a) in chondrogenesis and cartilage degeneration. Human adipose‑derived mesenchymal stem cells (hADSCs) in micromass and chondrocyte‑like ATDC5 cells were induced to chondrogenesis, and primary human/mouse chondrocytes (PHCs/PMCs) and chondrogenic ATDC5 cells were stimulated with interleukin‑1β (IL‑1β). An miR‑92a mimic/inhibitor was transfected into the ATDC5 cells using lipofectamine 2000. Gene expression was analyzed using reverse transcription‑quantitative polymerase chain reaction. Alcian blue was used to stain the cartilage nodules and chondrogenic micromass. The potential target genes, signaling pathways and functions of miR‑92a were examined using miRanda, miRDB, CLIP‑Seq, TargetScan and Kyoto Encyclopedia of Genes and Genomes. The expression of miR‑92a was elevated in the chondrogenic ATDC5 cells and hADSCs, and also in the IL‑1β‑induced ATDC5 cells, PMCs and PHCs. Forced expression of miR‑92a enhanced the expression levels of col9a2 and aggrecan. A total of 279 genes were predicted as potential target genes of miR‑92a. The phosphoinositide 3‑kinase/PI3K)‑Akt, ErbB and focal adhesion kinase pathways, extracellular matrix (ECM)‑receptor interaction and the mammalian target of rapamycin (mTOR) signaling pathway were suggested to mediate the effects of miR‑92a on chondrogenesis and cartilage degeneration. These results demonstrated that miR‑92a was involved in chondrogenesis and the chondrocyte response induced by IL‑1β. miR‑92a positively contributed to the expression of col9a2 and of aggrecan.
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